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Michael Pyne
Michael Pyne
Bestätigte E-Mail-Adresse bei uwo.ca
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Coupling the CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia coli
ME Pyne, M Moo-Young, DA Chung, CP Chou
Applied and environmental microbiology 81 (15), 5103-5114, 2015
2772015
Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium
ME Pyne, MR Bruder, M Moo-Young, DA Chung, CP Chou
Scientific reports 6 (1), 25666, 2016
2272016
Biochemical and genetic engineering strategies to enhance hydrogen production in photosynthetic algae and cyanobacteria
K Srirangan, ME Pyne, CP Chou
Bioresource technology 102 (18), 8589-8604, 2011
2002011
Engineering plant secondary metabolism in microbial systems
ME Pyne, L Narcross, VJJ Martin
Plant physiology 179 (3), 844-861, 2019
1822019
A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
ME Pyne, K Kevvai, PS Grewal, L Narcross, B Choi, L Bourgeois, ...
Nature Communications 11 (1), 3337, 2020
1472020
Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum
ME Pyne, M Moo-Young, DA Chung, CP Chou
Biotechnology for biofuels 6, 1-20, 2013
1122013
Technical guide for genetic advancement of underdeveloped and intractable Clostridium
ME Pyne, M Bruder, M Moo-Young, DA Chung, CP Chou
Biotechnology advances 32 (3), 623-641, 2014
982014
Extending CRISPR-Cas9 technology from genome editing to transcriptional engineering in the genus Clostridium
MR Bruder, ME Pyne, M Moo-Young, DA Chung, CP Chou
Applied and environmental microbiology 82 (20), 6109-6119, 2016
932016
A highly characterized synthetic landing pad system for precise multicopy gene integration in yeast
L Bourgeois, ME Pyne, VJJ Martin
ACS Synthetic Biology 7 (11), 2675-2685, 2018
622018
Biochemical, genetic, and metabolic engineering strategies to enhance coproduction of 1-propanol and ethanol in engineered Escherichia coli
K Srirangan, X Liu, A Westbrook, L Akawi, ME Pyne, M Moo-Young, ...
Applied microbiology and biotechnology 98, 9499-9515, 2014
552014
An Engineered Aro1 Protein Degradation Approach for Increased cis,cis-Muconic Acid Biosynthesis in Saccharomyces cerevisiae
ME Pyne, L Narcross, M Melgar, K Kevvai, S Mookerjee, GB Leite, ...
Applied and environmental microbiology 84 (17), e01095-18, 2018
542018
Disruption of the reductive 1, 3-propanediol pathway triggers production of 1, 2-propanediol for sustained glycerol fermentation by Clostridium pasteurianum
ME Pyne, S Sokolenko, X Liu, K Srirangan, MR Bruder, MG Aucoin, ...
Applied and environmental microbiology 82 (17), 5375-5388, 2016
412016
Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme
ME Pyne, M Moo-Young, DA Chung, CP Chou
Biotechnology for biofuels 7, 1-15, 2014
372014
Developing an Extended Genomic Engineering Approach Based on Recombineering to Knock-in Heterologous Genes to Escherichia coli Genome
K Sukhija, M Pyne, S Ali, V Orr, D Abedi, M Moo-Young, CP Chou
Molecular biotechnology 51, 109-118, 2012
342012
Using the Endogenous CRISPR-Cas System of Heliobacterium modesticaldum To Delete the Photochemical Reaction Center Core Subunit Gene
PL Baker, GS Orf, K Kevershan, ME Pyne, T Bicer, KE Redding
Applied and environmental microbiology 85 (23), e01644-19, 2019
222019
Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum
ME Pyne, X Liu, M Moo-Young, DA Chung, CP Chou
Scientific Reports 6 (1), 26228, 2016
222016
Pathway elucidation and microbial synthesis of proaporphine and bis-benzylisoquinoline alkaloids from sacred lotus (Nelumbo nucifera)
ME Pyne, ND Gold, VJJ Martin
Metabolic Engineering 77, 162-173, 2023
182023
Antisense-RNA-Mediated Gene Downregulation in Clostridium pasteurianum
ME Pyne, M Moo-Young, DA Chung, CP Chou
Fermentation 1 (1), 113-126, 2015
182015
Microbial synthesis of natural, semisynthetic, and new-to-nature tetrahydroisoquinoline alkaloids
ME Pyne, VJJ Martin
Current Opinion in Green and Sustainable Chemistry 33, 100561, 2022
162022
Improved draft genome sequence of Clostridium pasteurianum strain ATCC 6013 (DSM 525) using a hybrid next-generation sequencing approach
ME Pyne, S Utturkar, SD Brown, M Moo-Young, DA Chung, CP Chou
Genome announcements 2 (4), 10.1128/genomea. 00790-14, 2014
162014
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