SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities E Martínez-García, T Aparicio, A Goñi-Moreno, S Fraile, V de Lorenzo Nucleic acids research 43 (D1), D1183-D1189, 2015 | 237 | 2015 |
SEVA 3.0: an update of the Standard European Vector Architecture for enabling portability of genetic constructs among diverse bacterial hosts E Martínez-García, A Goni-Moreno, B Bartley, J McLaughlin, ... Nucleic acids research 48 (D1), D1164-D1170, 2020 | 213 | 2020 |
Selection of single domain antibodies from immune libraries displayed on the surface of E. coli cells with two β-domains of opposite topologies V Salema, E Marín, R Martínez-Arteaga, D Ruano-Gallego, S Fraile, ... PloS one 8 (9), e75126, 2013 | 113 | 2013 |
SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes E Martínez-García, S Fraile, E Algar, T Aparicio, E Velázquez, B Calles, ... Nucleic Acids Research 51 (D1), D1558-D1567, 2023 | 59 | 2023 |
Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system D Ruano-Gallego, S Fraile, C Gutierrez, LÁ Fernández Microbial Cell Factories 18, 1-13, 2019 | 56 | 2019 |
Monitoring Intracellular Levels of XylR in Pseudomonas putida with a Single-Chain Antibody Specific for Aromatic-Responsive Enhancer-Binding Proteins S Fraile, F Roncal, LA Fernández, V de Lorenzo Journal of Bacteriology 183 (19), 5571-5579, 2001 | 44 | 2001 |
Monitoring biodegradative enzymes with nanobodies raised in Camelus dromedarius with mixtures of catabolic proteins O Zafra, S Fraile, C Gutiérrez, A Haro, AD Páez‐Espino, JI Jiménez, ... Environmental Microbiology 13 (4), 960-974, 2011 | 36 | 2011 |
Increasing Signal Specificity of the TOL Network of Pseudomonas putida mt-2 by Rewiring the Connectivity of the Master Regulator XylR A de Las Heras, S Fraile, V de Lorenzo Public Library of Science 8 (10), e1002963, 2012 | 30 | 2012 |
Naked Bacterium: Emerging Properties of a Surfome-Streamlined Pseudomonas putida Strain E Martínez-García, S Fraile, D Rodriguez Espeso, D Vecchietti, G Bertoni, ... ACS Synthetic Biology 9 (9), 2477-2492, 2020 | 29 | 2020 |
Secretion of proteins with dimerization capacity by the haemolysin type I transport system of Escherichia coli S Fraile, A Muñoz, V De Lorenzo, LA Fernández Molecular microbiology 53 (4), 1109-1121, 2004 | 28 | 2004 |
Engineering Tropism of Pseudomonas putida toward Target Surfaces through Ectopic Display of Recombinant Nanobodies S Fraile, M Briones, M Revenga-Parra, V De Lorenzo, E Lorenzo, ... ACS Synthetic Biology 10 (8), 2049-2059, 2021 | 11 | 2021 |
Phenotypic knockouts of selected metabolic pathways by targeting enzymes with camel-derived nanobodies (VHHs) JI Jiménez, S Fraile, O Zafra, V de Lorenzo Metabolic Engineering 30, 40-48, 2015 | 11 | 2015 |
NanoPad: an integrated platform for bacterial production of camel nanobodies aimed at detecting environmental biomarkers S Fraile, JI Jiménez, C Gutiérrez, V De Lorenzo Proteomics 13 (18-19), 2766-2775, 2013 | 11 | 2013 |
Characterisation of the Putative Effector Interaction Site of the Regulatory HbpR Protein from Pseudomonas azelaica by Site-Directed Mutagenesis C Vogne, H Bisht, S Arias, S Fraile, R Lal, JR van der Meer PloS one 6 (2), e16539, 2011 | 7 | 2011 |
41 Emerging Systems and Synthetic Biology Approaches to Hydrocarbon Biotechnology V de Lorenzo, SFJI Jiménez | 4 | 2010 |
The naked cell: emerging properties of a surfome-streamlined Pseudomonas putida strain E Martínez-García, S Fraile, DR Espeso, D Vecchietti, G Bertoni, ... bioRxiv, 2020.05. 17.100628, 2020 | 3 | 2020 |
Rationally rewiring the connectivity of the XylR/Pu regulatory node of the m-xylene degradation pathway in Pseudomonas putida A De Las Heras, E Martínez-García, MR Domingo-Sananes, S Fraile, ... Integrative biology 8 (4), 571-576, 2016 | 2 | 2016 |
Programming bacterial adhesion to functionalized surfaces through cellular display of recombinant nanobodies S Fraile, E Veiga, V de Lorenzo, E Martínez-García Methods in Microbiology 53, 123-141, 2023 | 1 | 2023 |
Rationally rewiring the connectivity of the XylR/Pu regulatory node of the m-xylene degradation pathway in Pseudomonas putida A Heras, E Martínez García, MR Domingo-Sananes, S Fraile, V Lorenzo Royal Society of Chemistry (UK), 2016 | | 2016 |
Characterisation of the Putative Effector Interaction Site of the Regulatory HbpR Protein from C Vogne, H Bisht, S Arias, S Fraile, R Lal | | 2011 |