| Glutaraldehyde in bio-catalysts design: A useful crosslinker and a versatile tool in enzyme immobilization O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres, RC Rodrigues, ... Rsc Advances 4 (4), 1583-1600, 2014 | 897 | 2014 |
| Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres, RC Rodrigues, ... Biotechnology advances 33 (5), 435-456, 2015 | 703 | 2015 |
| Importance of the support properties for immobilization or purification of enzymes JCS Santos, O Barbosa, C Ortiz, A Berenguer‐Murcia, RC Rodrigues, ... ChemCatChem 7 (16), 2413-2432, 2015 | 573 | 2015 |
| Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions RC Rodrigues, JJ Virgen-Ortíz, JCS Dos Santos, Á Berenguer-Murcia, ... Biotechnology advances 37 (5), 746-770, 2019 | 548 | 2019 |
| Novozym 435: the “perfect” lipase immobilized biocatalyst? C Ortiz, ML Ferreira, O Barbosa, JCS dos Santos, RC Rodrigues, ... Catalysis Science & Technology 9 (10), 2380-2420, 2019 | 538 | 2019 |
| Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties O Barbosa, R Torres, C Ortiz, Á Berenguer-Murcia, RC Rodrigues, ... Biomacromolecules 14 (8), 2433-2462, 2013 | 538 | 2013 |
| Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts JJ Virgen-Ortiz, JCS Dos Santos, Á Berenguer-Murcia, O Barbosa, ... Journal of materials chemistry B 5 (36), 7461-7490, 2017 | 293 | 2017 |
| Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica O Barbosa, R Torres, C Ortiz, R Fernandez-Lafuente Process Biochemistry 47 (8), 1220-1227, 2012 | 250 | 2012 |
| Chemical modification in the design of immobilized enzyme biocatalysts: drawbacks and opportunities N Rueda, JCS Dos Santos, C Ortiz, R Torres, O Barbosa, RC Rodrigues, ... The Chemical Record 16 (3), 1436-1455, 2016 | 225 | 2016 |
| 435: The “perfect” lipase immobilized biocatalyst? Catal C Ortiz, ML Ferreira, O Barbosa, JCS Dos Santos, RC Rodrigues, ... Sci. Technol 9 (10), 2380-2420, 2019 | 169 | 2019 |
| Improved performance of lipases immobilized on heterofunctional octyl-glyoxyl agarose beads N Rueda, JCS dos Santos, R Torres, C Ortiz, O Barbosa, ... RSC advances 5 (15), 11212-11222, 2015 | 143 | 2015 |
| Amination of enzymes to improve biocatalyst performance: Coupling genetic modification and physicochemical tools RC Rodrigues, O Barbosa, C Ortiz, Á Berenguer-Murcia, R Torres, ... RSC advances 4 (72), 38350-38374, 2014 | 127 | 2014 |
| Easy stabilization of interfacially activated lipases using heterofunctional divinyl sulfone activated-octyl agarose beads. Modulation of the immobilized enzymes by altering … TL de Albuquerque, N Rueda, JCS dos Santos, O Barbosa, C Ortiz, ... Process Biochemistry 51 (7), 865-874, 2016 | 113 | 2016 |
| Bovine trypsin immobilization on agarose activated with divinylsulfone: Improved activity and stability via multipoint covalent attachment JCS dos Santos, N Rueda, O Barbosa, M del Carmen Millán-Linares, ... Journal of Molecular Catalysis B: Enzymatic 117, 38-44, 2015 | 112 | 2015 |
| Evaluation of divinylsulfone activated agarose to immobilize lipases and to tune their catalytic properties JCS dos Santos, N Rueda, R Torres, O Barbosa, LRB Goncalves, ... Process Biochemistry 50 (6), 918-927, 2015 | 112 | 2015 |
| Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin JCS dos Santos, N Rueda, O Barbosa, JF Fernández-Sánchez, ... RSC advances 5 (27), 20639-20649, 2015 | 112 | 2015 |
| Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene–divinylbenzene beads AB Martins, JLR Friedrich, JC Cavalheiro, C Garcia-Galan, O Barbosa, ... Bioresource technology 134, 417-422, 2013 | 112 | 2013 |
| Optimized preparation of CALB-CLEAs by response surface methodology: The necessity to employ a feeder to have an effective crosslinking J Cruz, O Barbosa, RC Rodrigues, R Fernandez-Lafuente, R Torres, ... Journal of Molecular Catalysis B: Enzymatic 80, 7-14, 2012 | 97 | 2012 |
| Immobilization/stabilization of ficin extract on glutaraldehyde-activated agarose beads. Variables that control the final stability and activity in protein hydrolyses EH Siar, S Arana-Peña, O Barbosa, MN Zidoune, R Fernandez-Lafuente Catalysts 8 (4), 149, 2018 | 91 | 2018 |
| Immobilization of CALB on activated chitosan: Application to enzymatic synthesis in supercritical and near-critical carbon dioxide JCS Dos Santos, HL Bonazza, LJBL de Matos, EA Carneiro, O Barbosa, ... Biotechnology reports 14, 16-26, 2017 | 91 | 2017 |