| One‐step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment LM Grav, JS Lee, S Gerling, TB Kallehauge, AH Hansen, S Kol, GM Lee, ... Biotechnology journal 10 (9), 1446-1456, 2015 | 163 | 2015 |
| CRISPR/Cas9‐mediated genome engineering of CHO cell factories: Application and perspectives JS Lee, LM Grav, NE Lewis, H Faustrup Kildegaard Biotechnology journal 10 (7), 979-994, 2015 | 149 | 2015 |
| Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion JM Gutierrez, A Feizi, S Li, TB Kallehauge, H Hefzi, LM Grav, D Ley, ... Nature communications 11 (1), 68, 2020 | 122 | 2020 |
| Accelerated homology‐directed targeted integration of transgenes in Chinese hamster ovary cells via CRISPR/Cas9 and fluorescent enrichment JS Lee, LM Grav, LE Pedersen, GM Lee, HF Kildegaard Biotechnology and bioengineering 113 (11), 2518-2523, 2016 | 85 | 2016 |
| Minimizing clonal variation during mammalian cell line engineering for improved systems biology data generation LM Grav, D Sergeeva, JS Lee, I Marin de Mas, NE Lewis, MR Andersen, ... ACS Synthetic Biology 7 (9), 2148-2159, 2018 | 77 | 2018 |
| Multicopy targeted integration for accelerated development of high-producing Chinese hamster ovary cells D Sergeeva, GM Lee, LK Nielsen, LM Grav ACS Synthetic Biology 9 (9), 2546-2561, 2020 | 56 | 2020 |
| Systematic evaluation of site-specific recombinant gene expression for programmable mammalian cell engineering N Pristovsek, S Nallapareddy, LM Grav, H Hefzi, NE Lewis, P Rugbjerg, ... ACS synthetic biology 8 (4), 758-774, 2019 | 55 | 2019 |
| Comprehensive analysis of genomic safe harbors as target sites for stable expression of the heterologous gene in HEK293 cells S Shin, SH Kim, SW Shin, LM Grav, LE Pedersen, JS Lee, GM Lee ACS synthetic biology 9 (6), 1263-1269, 2020 | 48 | 2020 |
| Factors affecting the quality of therapeutic proteins in recombinant Chinese hamster ovary cell culture TK Ha, D Kim, CL Kim, LM Grav, GM Lee Biotechnology Advances 54, 107831, 2022 | 47 | 2022 |
| Assessing developability early in the discovery process for novel biologics ML Fernández-Quintero, A Ljungars, F Waibl, V Greiff, JT Andersen, ... MAbs 15 (1), 2171248, 2023 | 44 | 2023 |
| Versatile microscale screening platform for improving recombinant protein productivity in Chinese hamster ovary cells HG Hansen, CN Nilsson, AM Lund, S Kol, LM Grav, M Lundqvist, ... Scientific reports 5 (1), 18016, 2015 | 41 | 2015 |
| Application of CRISPR/Cas9 genome editing to improve recombinant protein production in CHO cells LM Grav, KJ la Cour Karottki, JS Lee, HF Kildegaard Heterologous Protein Production in CHO Cells: Methods and Protocols, 101-118, 2017 | 33 | 2017 |
| An optimized genome-wide, virus-free CRISPR screen for mammalian cells K Xiong, KJ la Cour Karottki, H Hefzi, S Li, LM Grav, S Li, P Spahn, JS Lee, ... Cell Reports Methods 1 (4), 2021 | 30 | 2021 |
| Modular 5′-UTR hexamers for context-independent tuning of protein expression in eukaryotes SD Petersen, J Zhang, JS Lee, T Jakočiūnas, LM Grav, HF Kildegaard, ... Nucleic acids research 46 (21), e127-e127, 2018 | 28 | 2018 |
| BCAT1 and BCAT2 disruption in CHO cells has cell line-dependent effects S Pereira, D Ley, M Schubert, LM Grav, HF Kildegaard, MR Andersen Journal of Biotechnology 306, 24-31, 2019 | 10 | 2019 |
| Expanding the CRISPR toolbox for Chinese hamster ovary cells with comprehensive tools for Mad7 genome editing JB Rojek, Y Basavaraju, S Nallapareddy, DB Bulté, R Baumgartner, ... Biotechnology and Bioengineering 120 (6), 1478-1491, 2023 | 9 | 2023 |
| Antibody-dependent enhancement of toxicity of myotoxin II from Bothrops asper CV Sørensen, J Fernández, AC Adams, HHK Wildenauer, S Schoffelen, ... Nature Communications 15 (1), 173, 2024 | 8 | 2024 |
| Enhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free medium TK Ha, A Òdena, KJC Karottki, CL Kim, H Hefzi, GM Lee, ... Biotechnology and Bioengineering 120 (4), 1159-1166, 2023 | 7 | 2023 |
| Identification of hyperosmotic stress-responsive genes in Chinese hamster ovary cells via genome-wide virus-free CRISPR/Cas9 screening SH Kim, S Shin, M Baek, K Xiong, KJ la Cour Karottki, H Hefzi, LM Grav, ... Metabolic Engineering 80, 66-77, 2023 | 5 | 2023 |
| Characterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants CM Moore, A Ljungars, MJ Paul, CH Dahl, S Ahmadi, AC Adams, LM Grav, ... Toxicon 232, 107225, 2023 | 2 | 2023 |
Gyun Min LeeProfessor of Biological Sciences, KAISTkaist.ac.kr üzerinde doğrulanmış e-posta adresine sahip