| Pseudomonas 2.0: genetic upgrading of P. putida KT2440 as an enhanced host for heterologous gene expression E Martínez-García, PI Nikel, T Aparicio, V de Lorenzo Microbial cell factories 13, 1-15, 2014 | 268 | 2014 |
| SEVA 2.0: an update of the Standard European Vector Architecture for de-/re-construction of bacterial functionalities E Martínez-García, T Aparicio, A Goñi-Moreno, S Fraile, V de Lorenzo Nucleic acids research 43 (D1), D1183-D1189, 2015 | 236 | 2015 |
| CRISPR/Cas9‐Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida T Aparicio, V de Lorenzo, E Martínez‐García Biotechnology journal 13 (5), 1700161, 2018 | 155 | 2018 |
| New transposon tools tailored for metabolic engineering of Gram-negative microbial cell factories E Martínez-García, T Aparicio, V de Lorenzo, PI Nikel Frontiers in bioengineering and biotechnology 2, 46, 2014 | 137 | 2014 |
| Molecular characterization of the safracin biosynthetic pathway from Pseudomonas fluorescens A2‐2: designing new cytotoxic compounds A Velasco, P Acebo, A Gomez, C Schleissner, P Rodríguez, T Aparicio, ... Molecular microbiology 56 (1), 144-154, 2005 | 103 | 2005 |
| The Ssr protein (T1E_1405) from Pseudomonas putida DOT‐T1E enables oligonucleotide‐based recombineering in platform strain P. putida EM42 T Aparicio, SI Jensen, AT Nielsen, V de Lorenzo, E Martínez‐García Biotechnology journal 11 (10), 1309-1319, 2016 | 71 | 2016 |
| SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes E Martínez-García, S Fraile, E Algar, T Aparicio, E Velázquez, B Calles, ... Nucleic Acids Research 51 (D1), D1558-D1567, 2023 | 58 | 2023 |
| A standardized workflow for surveying recombinases expands bacterial genome‐editing capabilities DE Ricaurte, E Martínez‐García, A Nyerges, C Pal, V de Lorenzo, ... Microbial Biotechnology 11 (1), 176-188, 2018 | 40 | 2018 |
| High-efficiency multi-site genomic editing of Pseudomonas putida through thermoinducible ssDNA recombineering T Aparicio, A Nyerges, E Martínez-García, V de Lorenzo Iscience 23 (3), 2020 | 39 | 2020 |
| CRISPR/Cas9‐enhanced ssDNA recombineering for Pseudomonas putida T Aparicio, V de Lorenzo, E Martínez‐García Microbial Biotechnology 12 (5), 1076-1089, 2019 | 38 | 2019 |
| Engineering Gram-negative microbial cell factories using transposon vectors E Martínez-García, T Aparicio, V de Lorenzo, PI Nikel In Vitro Mutagenesis: Methods and Protocols, 273-293, 2017 | 33 | 2017 |
| Improved Thermotolerance of Genome‐Reduced Pseudomonas putida EM42 Enables Effective Functioning of the PL/cI857 System T Aparicio, V de Lorenzo, E Martínez‐García Biotechnology Journal 14 (1), 1800483, 2019 | 31 | 2019 |
| Broadening the SEVA plasmid repertoire to facilitate genomic editing of Gram-negative bacteria T Aparicio, V de Lorenzo, E Martínez-García Hydrocarbon and lipid microbiology protocols: genetic, genomic and system …, 2017 | 27 | 2017 |
| Mismatch repair hierarchy of Pseudomonas putida revealed by mutagenic ssDNA recombineering of the pyrF gene T Aparicio, A Nyerges, I Nagy, C Pal, E Martínez‐García, V de Lorenzo Environmental microbiology 22 (1), 45-58, 2020 | 21 | 2020 |
| Multifunctional SEVA shuttle vectors for actinomycetes and Gram‐negative bacteria C García‐Gutiérrez, T Aparicio, L Torres‐Sánchez, E Martínez‐García, ... Microbiologyopen 9 (6), 1135-1149, 2020 | 19 | 2020 |
| Functional mapping of PHF6 complexes in chromatin remodeling, replication dynamics, and DNA repair S Alvarez, AC da Silva Almeida, R Albero, M Biswas, A Barreto-Galvez, ... Blood, The Journal of the American Society of Hematology 139 (23), 3418-3429, 2022 | 13 | 2022 |
| Refactoring the conjugation machinery of promiscuous plasmid RP4 into a device for conversion of gram-negative isolates to Hfr strains J Silbert, V Lorenzo, T Aparicio ACS Synthetic Biology 10 (4), 690-697, 2021 | 10 | 2021 |
| A broad host range plasmid-based roadmap for ssDNA-based recombineering in Gram-negative bacteria T Aparicio, V de Lorenzo, E Martínez-García Horizontal Gene Transfer: Methods and Protocols, 383-398, 2020 | 10 | 2020 |
| An automated DIY framework for experimental evolution of Pseudomonas putida DR Espeso, P Dvořák, T Aparicio, V de Lorenzo Microbial Biotechnology 14 (6), 2679-2685, 2021 | 9 | 2021 |
| Reflection on the challenges, accomplishments, and new frontiers of gene drives M VergaraMichael BioDesign Research, 2022 | 8 | 2022 |
