CRISPR-based metabolic engineering in non-model microorganisms

L Lu, X Shen, X Sun, Y Yan, J Wang, Q Yuan - Current opinion in …, 2022 - Elsevier
Highlights•Non-model microorganisms serve as promising industrial chassis for
bioproduction.•CRISPR-based genetic modifications enable desired production …

Rewiring of metabolic pathways in yeasts for sustainable production of biofuels

R Maurya, N Gohil, S Nixon, N Kumar… - Bioresource …, 2023 - Elsevier
The ever-increasing global energy demand has led world towards negative repercussions
such as depletion of fossil fuels, pollution, global warming and climate change. Designing …

Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph

C Zhan, X Li, G Lan, EEK Baidoo, Y Yang, Y Liu… - Nature Catalysis, 2023 - nature.com
Methanol, an organic one-carbon (C1) compound, represents an attractive alternative
carbon source for microbial fermentation. Despite considerable advancements in methanol …

Engineering Rhodosporidium toruloides for production of 3-hydroxypropionic acid from lignocellulosic hydrolysate

D Liu, HJ Hwang, PB Otoupal, GM Geiselman… - Metabolic …, 2023 - Elsevier
Microbial production of valuable bioproducts is a promising route towards green and
sustainable manufacturing. The oleaginous yeast, Rhodosporidium toruloides, has emerged …

Dual Regulation of Cytoplasm and Peroxisomes for Improved Α-Farnesene Production in Recombinant Pichia pastoris

H Liu, SL Chen, JZ Xu, WG Zhang - ACS Synthetic Biology, 2021 - ACS Publications
Microbial production of α-farnesene from renewable raw materials is a feasible alternative to
traditional petroleum craft. Recently, the research on improving α-farnesene production in …

[HTML][HTML] Design and construction of microbial cell factories based on systems biology

W Yan, Z Cao, M Ding, Y Yuan - Synthetic and systems biotechnology, 2023 - Elsevier
Environmental sustainability is an increasingly important issue in industry. As an
environmentally friendly and sustainable way, constructing microbial cell factories to …

Improved polyketide production in C. glutamicum by preventing propionate-induced growth inhibition

C Zhan, N Lee, G Lan, Q Dan, A Cowan, Z Wang… - Nature …, 2023 - nature.com
Corynebacterium glutamicum is a promising host for production of valuable polyketides.
Propionate addition, a strategy known to increase polyketide production by increasing …

Metabolic engineering of Saccharomyces cerevisiae for the synthesis of valuable chemicals

S Wang, F Zhao, M Yang, Y Lin… - Critical Reviews in …, 2024 - Taylor & Francis
In the twenty first century, biotechnology offers great opportunities and solutions to climate
change mitigation, energy and food security and resource efficiency. The use of metabolic …

Key enzymes involved in the utilization of fatty acids by Saccharomyces cerevisiae: a review

Z Wang, C Su, Y Zhang, S Shangguan… - Frontiers in …, 2024 - frontiersin.org
Saccharomyces cerevisiae is a eukaryotic organism with a clear genetic background and
mature gene operating system; in addition, it exhibits environmental tolerance. Therefore, S …

[HTML][HTML] Genome-scale and pathway engineering for the sustainable aviation fuel precursor isoprenol production in Pseudomonas putida

D Banerjee, IS Yunus, X Wang, J Kim, A Srinivasan… - Metabolic …, 2024 - Elsevier
Sustainable aviation fuel (SAF) will significantly impact global warming in the aviation sector,
and important SAF targets are emerging. Isoprenol is a precursor for a promising SAF …