Investigating protein–protein interactions is crucial for understanding cellular biological
processes because proteins often function within molecular complexes rather than in …

What to measure? is a key question in nanoscience, and it is not straightforward to address
as different physicochemical properties define a nanoparticle sample. Most prominent …

BACKGROUND Biomolecular mechanisms are typically inferred from static structural
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …

Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of
molecular dynamics, and it has been widely applied in diverse fields, eg, biomedicine …

The field of fluorogenic RNA aptamers is a burgeoning research area that aims to address
the lack of naturally fluorescent RNA molecules for RNA detection and imaging. These small …

A ubiquitous observation in cell biology is that the diffusive motion of macromolecules and
organelles is anomalous, and a description simply based on the conventional diffusion …

Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

Providing much-needed information on fluorescence spectroscopy and microscopy, this
ready reference covers detection techniques, data registration, and the use of spectroscopic …

Single-molecule fluorescence techniques,, are key for a number of applications, including
DNA sequencing,, molecular and cell biology, and early diagnosis. Unfortunately …

In recent years fluorescence correlation spectroscopy (FCS) has become a routine method
for determining diffusion coefficients, chemical rate constants, molecular concentrations …