Fluorescent proteins and their applications in imaging living cells and tissues

DM Chudakov, MV Matz, S Lukyanov… - Physiological …, 2010 - journals.physiology.org
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

Chromophore transformations in red fluorescent proteins

FV Subach, VV Verkhusha - Chemical reviews, 2012 - ACS Publications
1. INTRODUCTION The discovery of Anthozoa homologues of the green fluorescent protein
(GFP) from jellyfish Aequorea victoria, which emit not only green but also yellow, orange …

Conversion of red fluorescent protein into a bright blue probe

OM Subach, IS Gundorov, M Yoshimura, FV Subach… - Chemistry & biology, 2008 - cell.com
We used a red chromophore formation pathway, in which the anionic red chromophore is
formed from the neutral blue intermediate, to suggest a rational design strategy to develop …

Structural basis for phototoxicity of the genetically encoded photosensitizer KillerRed

S Pletnev, NG Gurskaya, NV Pletneva… - Journal of Biological …, 2009 - ASBMB
KillerRed is the only known fluorescent protein that demonstrates notable phototoxicity,
exceeding that of the other green and red fluorescent proteins by at least 1,000-fold …

Light-dependent regulation of structural flexibility in a photochromic fluorescent protein

H Mizuno, TK Mal, M Wälchli… - Proceedings of the …, 2008 - National Acad Sciences
The structural basis for the photochromism in the fluorescent protein Dronpa is poorly
understood, because the crystal structures of the bright state of the protein did not provide an …

Beta-barrel scaffold of fluorescent proteins: folding, stability and role in chromophore formation

OV Stepanenko, OV Stepanenko, IM Kuznetsova… - International review of …, 2013 - Elsevier
This review focuses on the current view of the interaction between the β-barrel scaffold of
fluorescent proteins and their unique chromophore located in the internal helix. The …

Bond selection in the photoisomerization reaction of anionic green fluorescent protein and kindling fluorescent protein chromophore models

S Olsen, SC Smith - Journal of the American Chemical Society, 2008 - ACS Publications
The chromophores of the most widely known fluorescent proteins (FPs) are derivatives of a
core p-hydroxybenzylidene-imidazolinon-5-one (HBI) motif, which usually occurs as a …

A Crystallographic Study of Bright Far-Red Fluorescent Protein mKate Reveals pH-induced cis-trans Isomerization of the Chromophore∗

S Pletnev, D Shcherbo, DM Chudakov… - Journal of Biological …, 2008 - ASBMB
The far-red fluorescent protein mKate (λ ex, 588 nm; λ em, 635 nm; chromophore-forming
triad Met 63-Tyr 64-Gly 65), originating from wild-type red fluorescent progenitor eqFP578 …

[HTML][HTML] Structural characterization of acylimine-containing blue and red chromophores in mTagBFP and TagRFP fluorescent proteins

OM Subach, VN Malashkevich, WD Zencheck… - Chemistry & biology, 2010 - cell.com
We determined the 2.2 Å crystal structures of the red fluorescent protein TagRFP and its
derivative, the blue fluorescent protein mTagBFP. The crystallographic analysis is consistent …

Challenging a preconception: optoacoustic spectrum differs from the optical absorption spectrum of proteins and dyes for molecular imaging

JP Fuenzalida Werner, Y Huang, K Mishra… - Analytical …, 2020 - ACS Publications
Optoacoustic (photoacoustic) imaging has seen marked advances in detection and data
analysis, but there is less progress in understanding the photophysics of common …