A number of factors contribute to cell type–specific CTCF chromatin binding, but how they
act in concert to determine binding stability and functionality has not been fully elucidated. In …

Pioneer factors are transcription factors capable of binding to nucleosomal DNA, initiating
chromatin opening, and facilitating gene expression. By overcoming nucleosomes, pioneer …

Transcription factors guide tissue development by binding to developmental stage-specific
targets and establishing an appropriate enhancer landscape. In turn, DNA and chromatin …

Background Multiple enhancers co-regulating the same gene is prevalent and plays a
crucial role during development and disease. However, how multiple enhancers coordinate …

Master hematopoietic transcription factors (TFs) and long noncoding RNAs (lncRNAs)
coordinate sha** lineage-specific gene expression programs during hematopoietic …

We present SiCLAT, which introduces a dCas9-dCas13d cassette into the mouse genome.
This model enables the stable expression of both dCas9 and dCas13 proteins in diverse cell …

Eukaryotic genomes are replicated in a defined temporal order called the replication timing
(RT) program. RT is developmentally regulated, and has potential to drive cell fate …

Precise spatiotemporal and cell type-specific gene expression is essential for proper tissue
development and function. Transcription factors (TFs) guide this process by binding to …

MYOD is an E-box sequence-specific basic Helix-Loop-Helix (bHLH) transcriptional
activator that, when expressed in non-muscle cells, induces nuclear reprogramming toward …

CTCF binding sites serve as anchors for the 3D chromatin architecture in vertebrates. The
functionality of these anchors is influenced by the residence time of CTCF on chromatin …