Overcoming barriers to medium-chain fatty alcohol production

Y Su, AM Mangus, WT Cordell, BF Pfleger - Current opinion in …, 2024 - Elsevier
Highlights•mc FaOHs are valuable oleochemicals that demand development of a
sustainable source..•Substrate selectivity and product toxicity hinder microbial mc FaOH …

Conformational changes of acyl carrier protein switch the chain length preference of acyl-ACP thioesterase ChFatB2

T Yang, Y Yang, M Yang, J Ren, C Xue, Y Feng… - International Journal of …, 2023 - mdpi.com
Microbial fatty acids are synthesized by Type II fatty acid synthase and could be tailored by
acyl-ACP thioesterase. With the prospects of medium-chain fatty-acid-derivative biofuels, the …

MALDI-MS screening of microbial colonies with isomer resolution to select fatty acid desaturase variants

K Choe, MA **dra, SC Hubbard, BF Pfleger… - Analytical …, 2023 - Elsevier
Creating controlled lipid unsaturation locations in oleochemicals can be a key to many
bioengineered products. However, evaluating the effects of modifications to the acyl-ACP …

A Fast-Pass, Desorption Electrospray Ionization Mass Spectrometry Strategy for Untargeted Metabolic Phenoty**

HS Shepard, JC May, BE Zuniga… - Journal of the …, 2025 - ACS Publications
Desorption electrospray ionization mass spectrometry imaging (DESI-MSI) provides direct
analytical readouts of small molecules that can be used to characterize the metabolic …

Characterization of acyl-lipid thioesterase (ALT) enzymes from plants

RS Kalinger - 2023 - repository.library.carleton.ca
Abstract Medium-chain (6-14 carbon) fatty acids and their oleochemical derivatives are high-
value compounds used in the fuel, food, pharmaceutical, and plastics industries, among …

Workflow for High-throughput Screening of Enzyme Mutant Libraries Using Matrix-assisted Laser Desorption/Ionization Mass Spectrometry Analysis of Escherichia coli …

K Choe, JV Sweedler - Bio-protocol, 2023 - bio-protocol.org
Background Directed evolution is an iterative process of mutagenesis and phenotype
screening to achieve incremental improvements towards desired characteristics (Arnold …

[引用][C] Bio-Based Commodity Chemicals Via Genetically Engineered Microbes

S Mishra, PM Perkovich, BF Pfleger - 2024 - Elsevier