Caspases and their substrates

O Julien, JA Wells - Cell Death & Differentiation, 2017 - nature.com
Protease biology is intimately linked to the functional consequences of substrate cleavage
events. Human caspases are a family of 12 fate-determining cysteine proteases that are best …

A deep proteome and transcriptome abundance atlas of 29 healthy human tissues

D Wang, B Eraslan, T Wieland, B Hallström… - Molecular systems …, 2019 - embopress.org
Abstract Genome‐, transcriptome‐and proteome‐wide measurements provide insights into
how biological systems are regulated. However, fundamental aspects relating to which …

An optimized shotgun strategy for the rapid generation of comprehensive human proteomes

DB Bekker-Jensen, CD Kelstrup, TS Batth, SC Larsen… - Cell systems, 2017 - cell.com
This study investigates the challenge of comprehensively cataloging the complete human
proteome from a single-cell type using mass spectrometry (MS)-based shotgun proteomics …

Deorphanizing caspase-3 and caspase-9 substrates in and out of apoptosis with deep substrate profiling

LE Araya, IV Soni, JA Hardy, O Julien - ACS chemical biology, 2021 - ACS Publications
Caspases are a family of enzymes that regulate biological processes such as inflammation
and programmed cell death, through proteolysis. For example, in the intrinsic pathway of …

Ten years of extracellular matrix proteomics: accomplishments, challenges, and future perspectives

A Naba - Molecular & Cellular Proteomics, 2023 - ASBMB
The extracellular matrix (ECM) is a complex assembly of hundreds of proteins forming the
architectural scaffold of multicellular organisms. In addition to its structural role, the ECM …

Proteolytic ectodomain shedding of membrane proteins in mammals—hardware, concepts, and recent developments

SF Lichtenthaler, MK Lemberg, R Fluhrer - The EMBO journal, 2018 - embopress.org
Proteolytic removal of membrane protein ectodomains (ectodomain shedding) is a post‐
translational modification that controls levels and function of hundreds of membrane …

Proteolytic networks in cancer

SD Mason, JA Joyce - Trends in cell biology, 2011 - cell.com
Proteases are important for multiple processes during malignant progression, including
tumor angiogenesis, invasion and metastasis. Recent evidence reveals that tumor …

Understanding fibroblast activation protein (FAP): substrates, activities, expression and targeting for cancer therapy

EJ Hamson, FM Keane, S Tholen… - PROTEOMICS …, 2014 - Wiley Online Library
Fibroblast activation protein (FAP) is best known for its heightened expression in tumour
stroma. This atypical serine protease has both dipeptidyl peptidase and endopeptidase …

Characterising proteolysis during SARS-CoV-2 infection identifies viral cleavage sites and cellular targets with therapeutic potential

B Meyer, J Chiaravalli, S Gellenoncourt… - Nature …, 2021 - nature.com
SARS-CoV-2 is the causative agent behind the COVID-19 pandemic, responsible for over
170 million infections, and over 3.7 million deaths worldwide. Efforts to test, treat and …

Effective isolation of exosomes with polyethylene glycol from cell culture supernatant for in-depth proteome profiling

Y Weng, Z Sui, Y Shan, Y Hu, Y Chen, L Zhang… - Analyst, 2016 - pubs.rsc.org
Exosomes are secreted nanovesicles shed by almost all kinds of cells. Recently, increased
interest has been focused on these extracellular vesicles as natural carriers transporting …