Pseudomonas putida KT2440: the long journey of a soil-dweller to become a synthetic biology chassis

V de Lorenzo, D Pérez-Pantoja, PI Nikel - Journal of bacteriology, 2024 - journals.asm.org
Although members of the genus Pseudomonas share specific morphological, metabolic, and
genomic traits, the diversity of niches and lifestyles adopted by the family members is vast …

CRISPR/Cas9‐Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida

T Aparicio, V de Lorenzo… - Biotechnology …, 2018 - Wiley Online Library
While adoption of single‐stranded DNA recombineering techniques has greatly eased
genetic design of the platform strain Pseudomonas putida KT2440, available methods still …

[HTML][HTML] Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida

DC Volke, L Friis, NT Wirth, J Turlin, PI Nikel - Metabolic engineering …, 2020 - Elsevier
Genome engineering of non-conventional microorganisms calls for the development of
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …

Contextual dependencies expand the re-usability of genetic inverters

H Tas, L Grozinger, R Stoof, V de Lorenzo… - Nature …, 2021 - nature.com
The implementation of Boolean logic circuits in cells have become a very active field within
synthetic biology. Although these are mostly focussed on the genetic components alone, the …

Recent advances in genetic tools for engineering probiotic lactic acid bacteria

K Mugwanda, S Hamese, WF Van Zyl… - Bioscience …, 2023 - portlandpress.com
Synthetic biology has grown exponentially in the last few years, with a variety of biological
applications. One of the emerging applications of synthetic biology is to exploit the link …

[HTML][HTML] Engineering and modification of microbial chassis for systems and synthetic biology

H Chi, X Wang, Y Shao, Y Qin, Z Deng, L Wang… - Synthetic and Systems …, 2019 - Elsevier
Engineering and modifying synthetic microbial chassis is one of the best ways not only to
unravel the fundamental principles of life but also to enhance applications in the health …

Recent advances in synthetic biology of cyanobacteria

A Sengupta, HB Pakrasi, PP Wangikar - Applied microbiology and …, 2018 - Springer
Cyanobacteria are attractive hosts that can be engineered for the photosynthetic production
of fuels, fine chemicals, and proteins from CO 2. Moreover, the responsiveness of these …

Molecular tools and emerging strategies for deep genetic/genomic refactoring of Pseudomonas

E Martinez-Garcia, V de Lorenzo - Current opinion in biotechnology, 2017 - Elsevier
Highlights•Pseudomonas species are well suited as a platform for industrial and
environmental biotechnology.•A range of Synthetic Biology methods for genetic refactoring …

A framework for the systematic selection of biosensor chassis for environmental synthetic biology

S Sridhar, CM Ajo-Franklin, CA Masiello - ACS Synthetic Biology, 2022 - ACS Publications
Microbial biosensors sense and report exposures to stimuli, thereby facilitating our
understanding of environmental processes. Successful design and deployment of …

Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator)

L Santolin, SL Riedel, CJ Brigham - Applied Microbiology and …, 2024 - Springer
Synthetic biology encompasses many kinds of ideas and techniques with the common
theme of creating something novel. The industrially relevant microorganism, Ralstonia …