Experimental approaches to studying translation in plant semi-autonomous organelles

M Kwasniak-Owczarek, H Janska - Journal of Experimental …, 2024 - academic.oup.com
Plant mitochondria and chloroplasts are semi-autonomous organelles originated from free-
living bacteria that have retained reduced genomes during evolution. As a consequence …

Removal of the large inverted repeat from the plastid genome reveals gene dosage effects and leads to increased genome copy number

C Krämer, CR Boehm, J Liu, MKY Ting, AP Hertle… - Nature Plants, 2024 - nature.com
The chloroplast genomes of most plants and algae contain a large inverted repeat (IR)
region that separates two single-copy regions and harbours the ribosomal RNA operon. We …

Streamlining Protein Fractional Synthesis Rates Using SP3 Beads and Stable Isotope Mass Spectrometry: A Case Study on the Plant Ribosome

D Gentry-Torfer, E Murillo, CL Barrington, S Nie… - Bio …, 2024 - bio-protocol.org
Background Throughout the tree of life (TOL), ribosomes play a pivotal role in synthesizing
all proteins by decoding mRNA [3]. The most precise representations of the TOL to date …