Contamination in low microbial biomass microbiome studies: issues and recommendations
Next-generation sequencing approaches in microbiome research have allowed surveys of
microbial communities, their genomes, and their functions with higher sensitivity than ever …
microbial communities, their genomes, and their functions with higher sensitivity than ever …
Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea
We present two standards developed by the Genomic Standards Consortium (GSC) for
reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum …
reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum …
Aging and neurodegeneration are associated with increased mutations in single human neurons
It has long been hypothesized that aging and neurodegeneration are associated with
somatic mutation in neurons; however, methodological hurdles have prevented testing this …
somatic mutation in neurons; however, methodological hurdles have prevented testing this …
Sequencing-based analysis of microbiomes
Microbiomes occupy a range of niches and, in addition to having diverse compositions, they
have varied functional roles that have an impact on agriculture, environmental sciences, and …
have varied functional roles that have an impact on agriculture, environmental sciences, and …
Assembling single-cell genomes and mini-metagenomes from chimeric MDA products
Recent advances in single-cell genomics provide an alternative to largely gene-centric
metagenomics studies, enabling whole-genome sequencing of uncultivated bacteria …
metagenomics studies, enabling whole-genome sequencing of uncultivated bacteria …
Assembling genomes and mini-metagenomes from highly chimeric reads
Recent advances in single-cell genomics provide an alternative to gene-centric
metagenomics studies, enabling whole genome sequencing of uncultivated bacteria …
metagenomics studies, enabling whole genome sequencing of uncultivated bacteria …
Droplet microfluidics for high-throughput biological assays
Droplet microfluidics offers significant advantages for performing high-throughput screens
and sensitive assays. Droplets allow sample volumes to be significantly reduced, leading to …
and sensitive assays. Droplets allow sample volumes to be significantly reduced, leading to …
Single-Cell Genomics Reveals Hundreds of Coexisting Subpopulations in Wild Prochlorococcus
Extensive genomic diversity within coexisting members of a microbial species has been
revealed through selected cultured isolates and metagenomic assemblies. Yet, the cell-by …
revealed through selected cultured isolates and metagenomic assemblies. Yet, the cell-by …
Bacterial vesicles in marine ecosystems
Many heterotrophic bacteria are known to release extracellular vesicles, facilitating
interactions between cells and their environment from a distance. Vesicle production has not …
interactions between cells and their environment from a distance. Vesicle production has not …
[HTML][HTML] Genome-wide single-cell analysis of recombination activity and de novo mutation rates in human sperm
Meiotic recombination and de novo mutation are the two main contributions toward gamete
genome diversity, and many questions remain about how an individual human's genome is …
genome diversity, and many questions remain about how an individual human's genome is …