Direct transposition of native DNA for sensitive multimodal single-molecule sequencing

AS Nanda, K Wu, I Irkliyenko, B Woo, MS Ostrowski… - Nature Genetics, 2024 - nature.com
Concurrent readout of sequence and base modifications from long unamplified DNA
templates by Pacific Biosciences of California (PacBio) single-molecule sequencing …

Chasing Sequencing Perfection: Marching Toward Higher Accuracy and Lower Costs

H Jia, S Tan, YE Zhang - Genomics, Proteomics & Bioinformatics, 2024 - academic.oup.com
Abstract Next-generation sequencing (NGS), represented by Illumina platforms, has been an
essential cornerstone of basic and applied research. However, the sequencing error rate of …

Neuropathologically directed profiling of PRNP somatic and germline variants in sporadic human prion disease

GA McDonough, Y Cheng, KS Morillo, RN Doan… - Acta …, 2024 - Springer
Abstract Creutzfeldt–Jakob Disease (CJD), the most common human prion disease, is
associated with pathologic misfolding of the prion protein (PrP), encoded by the PRNP gene …

[HTML][HTML] Disentangling sources of clock-like mutations in germline and soma

N Spisak, M de Manuel, W Milligan, G Sella… - bioRxiv, 2023 - ncbi.nlm.nih.gov
The rates of mutations vary across cell types. To identify causes of this variation, mutations
are often decomposed into a combination of the single base substitution (SBS)“signatures” …

Ultra-sensitive molecular residual disease detection through whole genome sequencing with single-read error correction

X Li, T Liu, A Bacchiocchi, M Li, W Cheng… - EMBO Molecular …, 2024 - embopress.org
While whole genome sequencing (WGS) of cell-free DNA (cfDNA) holds enormous promise
for detection of molecular residual disease (MRD), its performance is limited by WGS error …

The clock-like accumulation of germline and somatic mutations can arise from the interplay of DNA damage and repair

N Spisak, M de Manuel, W Milligan, G Sella… - PLoS …, 2024 - journals.plos.org
The rates at which mutations accumulate across human cell types vary. To identify causes of
this variation, mutations are often decomposed into a combination of the single-base …

NeMu: a comprehensive pipeline for accurate reconstruction of neutral mutation spectra from evolutionary data

B Efimenko, K Popadin, K Gunbin - Nucleic Acids Research, 2024 - academic.oup.com
The recognized importance of mutational spectra in molecular evolution is yet to be fully
exploited beyond human cancer studies and model organisms. The wealth of intraspecific …