Characterizing cis-regulatory elements using single-cell epigenomics

S Preissl, KJ Gaulton, B Ren - Nature Reviews Genetics, 2023‏ - nature.com
Cell type-specific gene expression patterns and dynamics during development or in disease
are controlled by cis-regulatory elements (CREs), such as promoters and enhancers …

Breast cancer heterogeneity and its implication in personalized precision therapy

L Guo, D Kong, J Liu, L Zhan, L Luo, W Zheng… - … hematology & oncology, 2023‏ - Springer
Breast cancer heterogeneity determines cancer progression, treatment effects, and
prognosis. However, the precise mechanism for this heterogeneity remains unknown owing …

Recent advances in droplet microfluidics for single-cell analysis

Z Jiang, H Shi, X Tang, J Qin - TrAC Trends in Analytical Chemistry, 2023‏ - Elsevier
Single-cell manipulation and analysis is essential for studying many fundamental biological
processes and discovering cellular heterogeneity. Multi-parameter studies of intercellular …

[HTML][HTML] Single cell cancer epigenetics

M Casado-Pelaez, A Bueno-Costa, M Esteller - Trends in Cancer, 2022‏ - cell.com
Bulk sequencing methodologies have allowed us to make great progress in cancer
research. Unfortunately, these techniques lack the resolution to fully unravel the epigenetic …

Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity

Y Hu, F Shen, X Yang, T Han, Z Long, J Wen… - Clinical …, 2023‏ - Springer
Background Previous studies have traditionally attributed the initiation of cancer cells to
genetic mutations, considering them as the fundamental drivers of carcinogenesis. However …

Sample-multiplexing approaches for single-cell sequencing

Y Zhang, S Xu, Z Wen, J Gao, S Li… - Cellular and Molecular …, 2022‏ - Springer
Single-cell sequencing is widely used in biological and medical studies. However, its
application with multiple samples is hindered by inefficient sample processing, high …

MitoSort: Robust Demultiplexing of Pooled Single-cell Genomic Data Using Endogenous Mitochondrial Variants

Z Tang, W Zhang, P Shi, S Li, X Li, Y Li… - Genomics …, 2024‏ - academic.oup.com
Multiplexing across donors has emerged as a popular strategy to increase throughput,
reduce costs, overcome technical batch effects, and improve doublet detection in single-cell …

Highly sensitive single-cell chromatin accessibility assay and transcriptome coassay with METATAC

H Wu, X Li, F Jian, A Yisimayi… - Proceedings of the …, 2022‏ - National Acad Sciences
Recent advances in single-cell assay for transposase accessible chromatin using
sequencing (scATAC-seq) and its coassays have transformed the field of single-cell …

Cellular barcoding: From developmental tracing to anti-tumor drug discovery

Y Wang, X Zhang, Z Wang - Cancer Letters, 2023‏ - Elsevier
Clonal evolution has gained immense attention in explaining cancer cell status, history, and
fate during cancer progression. Current single-cell or spatial transcriptome technologies …

Challenges and considerations for single-cell and spatially resolved transcriptomics sample collection during spaceflight

EG Overbey, S Das, H Cope, P Madrigal… - Cell reports …, 2022‏ - cell.com
Single-cell RNA sequencing (scRNA-seq) and spatially resolved transcriptomics (SRT) have
experienced rapid development in recent years. The findings of spaceflight-based scRNA …