Biliary secretion of quasi-enveloped human hepatitis A virus

A Hirai-Yuki, L Hensley, JK Whitmire, SM Lemon - MBio, 2016 - Am Soc Microbiol
Hepatitis A virus (HAV) is an unusual picornavirus that is released from cells cloaked in host-
derived membranes. These quasi-enveloped virions (eHAV) are the only particle type …

Technological advancements for the development of stem cell-based models for hepatotoxicity testing

A Natale, K Vanmol, A Arslan, S Van Vlierberghe… - Archives of …, 2019 - Springer
Stem cells are characterized by their self-renewal capacity and their ability to differentiate
into multiple cell types of the human body. Using directed differentiation strategies, stem …

Vectorial release of hepatitis E virus in polarized human hepatocytes

N Capelli, O Marion, M Dubois, S Allart… - Journal of …, 2019 - Am Soc Microbiol
Hepatitis E virus (HEV) is a common cause of acute viral hepatitis worldwide. Most HEV
infections are asymptomatic, but immunocompromised patients infected with HEV genotype …

Interactions between the hepatitis C virus nonstructural 2 protein and host adaptor proteins 1 and 4 orchestrate virus release

F **ao, S Wang, R Barouch-Bentov, G Neveu, S Pu… - MBio, 2018 - Am Soc Microbiol
Hepatitis C virus (HCV) spreads via secreted cell-free particles or direct cell-to-cell
transmission. Yet, virus-host determinants governing differential intracellular trafficking of …

Entry and release of hepatitis C virus in polarized human hepatocytes

S Belouzard, A Danneels, L Fénéant, K Séron… - Journal of …, 2017 - Am Soc Microbiol
Hepatitis C virus (HCV) primarily infects hepatocytes, which are highly polarized cells. The
relevance of cell polarity in the HCV life cycle has been addressed only in distantly related …

A CRISPR screen identifies the cell polarity determinant crumbs 3 as an adeno-associated virus restriction factor in hepatocytes

VJ Madigan, TO Tyson, JA Yuziuk, M Pillai… - Journal of …, 2019 - Am Soc Microbiol
Adeno-associated viruses (AAV) are helper-dependent parvoviruses that have been
developed into promising gene therapy vectors. Many studies, including a recent unbiased …

The viral ORF3 protein is required for hepatitis E virus apical release and efficient growth in polarized hepatocytes and humanized mice

G Sari, J Zhu, C Ambardekar, X Yin, A Boonstra… - Journal of …, 2021 - Am Soc Microbiol
Hepatitis E virus (HEV), an enterically transmitted RNA virus, is a major cause of acute
hepatitis worldwide. Additionally, HEV genotype 3 (gt3) can frequently persist in …

Induction of Bile Canaliculi-Forming Hepatocytes from Human Pluripotent Stem Cells

L Matakovic, AW Overeem, K Klappe… - … : Methods and Protocols, 2022 - Springer
Cell polarity and formation of bile canaliculi can be achieved in hepatocytes which are
generated from patient-derived induced pluripotent stem cells. This allows for the study of …

Live-cell Imaging of Biosynthetic Protein Transport in Hepatocytes

F Lázaro-Diéguez, A Müsch - Hepatocytes: Methods and Protocols, 2022 - Springer
Here, we describe a strategy to analyze the exit of apical and basolateral cargo from the
trans-Golgi network in primary hepatocytes. The method is based on recombinant …