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Volume electron microscopy
Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …
methods provided only 2D image data. Recently, electron microscopy techniques capable of …
Volume EM: a quiet revolution takes shape
Volume electron microscopy (vEM) is a group of techniques that reveal the 3D ultrastructure
of cells and tissues through continuous depths of at least 1 micrometer. A burgeoning …
of cells and tissues through continuous depths of at least 1 micrometer. A burgeoning …
[HTML][HTML] Cryo-focused ion beam for in situ structural biology: State of the art, challenges, and perspectives
Cryogenic-focused ion beam (cryo-FIB) instruments became essential for high-resolution
imaging in cryo-preserved cells and tissues. Cryo-FIBs use accelerated ions to thin samples …
imaging in cryo-preserved cells and tissues. Cryo-FIBs use accelerated ions to thin samples …
[HTML][HTML] A modular platform for automated cryo-FIB workflows
Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB)
has matured into a preparation method widely used for cellular cryo-electron tomography …
has matured into a preparation method widely used for cellular cryo-electron tomography …
Correlative multi-scale cryo-imaging unveils SARS-CoV-2 assembly and egress
Since the outbreak of the SARS-CoV-2 pandemic, there have been intense structural studies
on purified viral components and inactivated viruses. However, structural and ultrastructural …
on purified viral components and inactivated viruses. However, structural and ultrastructural …
Cryo-plasma FIB/SEM volume imaging of biological specimens
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and
assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to …
assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to …
Structure and activity of particulate methane monooxygenase arrays in methanotrophs
Methane-oxidizing bacteria play a central role in greenhouse gas mitigation and have
potential applications in biomanufacturing. Their primary metabolic enzyme, particulate …
potential applications in biomanufacturing. Their primary metabolic enzyme, particulate …
[HTML][HTML] Super-resolution confocal cryo-CLEM with cryo-FIB milling for in situ imaging of Deinococcus radiodurans
Studying bacterial cell envelope architecture with electron microscopy is challenging due to
the poor preservation of microbial ultrastructure with traditional methods. Here, we …
the poor preservation of microbial ultrastructure with traditional methods. Here, we …
Recent advances in plant endomembrane research and new microscopical techniques
The endomembrane system consists of various membrane‐bound organelles including the
endoplasmic reticulum (ER), Golgi apparatus, trans‐Golgi network (TGN), endosomes, and …
endoplasmic reticulum (ER), Golgi apparatus, trans‐Golgi network (TGN), endosomes, and …
Disruption of the mitochondrial network in a mouse model of Huntington's disease visualized by in-tissue multiscale 3D electron microscopy
Huntington's disease (HD) is an inherited neurodegenerative disorder caused by an
expanded CAG repeat in the coding sequence of huntingtin protein. Initially, it predominantly …
expanded CAG repeat in the coding sequence of huntingtin protein. Initially, it predominantly …