Recent progress in DNA manipulation and gene circuit engineering has greatly improved
our ability to programme and probe mammalian cell behaviour. These advances have led to …

Mammalian cells have developed dedicated molecular mechanisms to tightly control
expression levels of their genes where the specific transcriptomic signature across all genes …

Genetic circuits and reaction cascades are of great importance for synthetic biology,
biochemistry and bioengineering. An open question is how to maximize the modularity of …

Methods for one-photon fluorescent imaging of calcium dynamics can capture the activity of
hundreds of neurons across large fields of view at a low equipment complexity and cost. In …

Protein interactions guide most cellular processes. Orthogonal hetero-specific protein–
protein interaction domains may facilitate better control of engineered biological systems …

Engineering mammalian cell-based devices that monitor and therapeutically modulate
human physiology is a promising and emerging frontier in clinical synthetic biology …

Intein-mediated protein splicing has become an essential tool in modern biotechnology.
Fundamental progress in the structure and catalytic strategies of cis-and trans-splicing …

A common strategy to understand a biological system is to selectively perturb it and observe
its response. Although technologies now exist to manipulate cellular systems at the genetic …

The ability to monitor and perturb RNAs in living cells would benefit greatly from a modular
protein architecture that targets unmodified RNA sequences in a programmable way. We …

We generated synthetic protein components that can detect specific DNA sequences and
subsequently trigger a desired intracellular response. These modular sensors exploit the …