Real-time PCR in clinical microbiology: applications for routine laboratory testing

MJ Espy, JR Uhl, LM Sloan… - Clinical microbiology …, 2006 - Am Soc Microbiol
Real-time PCR has revolutionized the way clinical microbiology laboratories diagnose many
human microbial infections. This testing method combines PCR chemistry with fluorescent …

[HTML][HTML] Real-time PCR in the microbiology laboratory

IM Mackay - Clinical microbiology and infection, 2004 - Elsevier
Use of PCR in the field of molecular diagnostics has increased to the point where it is now
accepted as the standard method for detecting nucleic acids from a number of sample and …

Prevalence and diversity of microbes in the amniotic fluid, the fetal inflammatory response, and pregnancy outcome in women with preterm pre‐labor rupture of …

DB DiGiulio, R Romero, JP Kusanovic… - American journal of …, 2010 - Wiley Online Library
Citation DiGiulio DB, Romero R, Kusanovic JP, Gómez R, Kim CJ, Seok K, Gotsch F, Mazaki‐
Tovi S, Vaisbuch E, Sanders K, Bik EM, Chaiworapongsa T, Oyarzún E, Relman DA …

New Real-Time PCR Assay for Rapid Detection of Methicillin- Resistant Staphylococcus aureus Directly from Specimens Containing a Mixture of Staphylococci

A Huletsky, R Giroux, V Rossbach… - Journal of clinical …, 2004 - Am Soc Microbiol
Molecular methods for the rapid identification of methicillin-resistant Staphylococcus aureus
(MRSA) are generally based on the detection of an S. aureus-specific gene target and the …

Development of a PCR assay for identification of staphylococci at genus and species levels

F Martineau, FJ Picard, D Ke, S Paradis… - Journal of clinical …, 2001 - Am Soc Microbiol
We have developed a PCR-based assay which allows the detection of staphylococci at the
genus level by targeting the tuf gene, which encodes the elongation factor Tu. Degenerate …

Rapid detection of group B streptococci in pregnant women at delivery

MG Bergeron, D Ke, C Ménard… - New England journal …, 2000 - Mass Medical Soc
Background Group B streptococcal infections are an important cause of neonatal morbidity
and mortality. A rapid method for the detection of this organism in pregnant women at the …

Occurrence of rare genotypes of Streptococcus agalactiae in cultured red tilapia Oreochromis sp. and Nile tilapia O. niloticus in Thailand—relationship to human …

N Suanyuk, F Kong, D Ko, GL Gilbert, K Supamattaya - Aquaculture, 2008 - Elsevier
Streptococcus agalactiae (Group B streptococcus, GBS) is an important pathogen affecting
humans and animals as well as aquatic species. Between April 2003 and March 2006 …

Rapid Detection of Clostridium difficile in Feces by Real-Time PCR

SD Bélanger, M Boissinot, N Clairoux… - Journal of clinical …, 2003 - Am Soc Microbiol
Clostridium difficile is the major causative agent of nosocomial antibiotic-associated
diarrhea, colitis, and pseudomembranous colitis. The pathogenicity of C. difficile is closely …

Bacterial pathogen detection by conventional culture‐based and recent alternative (polymerase chain reaction, isothermal amplification, enzyme linked …

SO Kim, SS Kim - Journal of Food Safety, 2021 - Wiley Online Library
The rapid, sensitive, and selective detection of foodborne pathogens is important to ensure
food safety. Culture medium‐based methods for bacteria detection have long been used …

Reliable detection of group B Streptococcus in the clinical laboratory

M Rosa-Fraile, B Spellerberg - Journal of clinical microbiology, 2017 - Am Soc Microbiol
Group B streptococcus (GBS) is a leading cause of invasive neonatal infections and a
significant pathogen in immunocompromised adults. Screening to detect GBS colonization …