Profiling cell identity and tissue architecture with single-cell and spatial transcriptomics

GS Gulati, JP D'Silva, Y Liu, L Wang… - … Reviews Molecular Cell …, 2024 - nature.com
Single-cell transcriptomics has broadened our understanding of cellular diversity and gene
expression dynamics in healthy and diseased tissues. Recently, spatial transcriptomics has …

[HTML][HTML] Automated methods for cell type annotation on scRNA-seq data

G Pasquini, JER Arias, P Schäfer… - Computational and …, 2021 - Elsevier
The advent of single-cell sequencing started a new era of transcriptomic and genomic
research, advancing our knowledge of the cellular heterogeneity and dynamics. Cell type …

YTHDF2 inhibition potentiates radiotherapy antitumor efficacy

L Wang, X Dou, S Chen, X Yu, X Huang, L Zhang… - Cancer Cell, 2023 - cell.com
Summary RNA N 6-methyladenosine (m 6 A) modification is implicated in cancer
progression. However, the impact of m 6 A on the antitumor effects of radiotherapy and the …

Benchmarking clustering algorithms on estimating the number of cell types from single-cell RNA-sequencing data

L Yu, Y Cao, JYH Yang, P Yang - Genome biology, 2022 - Springer
Background A key task in single-cell RNA-seq (scRNA-seq) data analysis is to accurately
detect the number of cell types in the sample, which can be critical for downstream analyses …

Data analysis guidelines for single-cell RNA-seq in biomedical studies and clinical applications

M Su, T Pan, QZ Chen, WW Zhou, Y Gong, G Xu… - Military Medical …, 2022 - Springer
The application of single-cell RNA sequencing (scRNA-seq) in biomedical research has
advanced our understanding of the pathogenesis of disease and provided valuable insights …

Spearheading future omics analyses using dyngen, a multi-modal simulator of single cells

R Cannoodt, W Saelens, L Deconinck… - Nature …, 2021 - nature.com
We present dyngen, a multi-modal simulation engine for studying dynamic cellular
processes at single-cell resolution. dyngen is more flexible than current single-cell …

Opportunities and challenges of single-cell and spatially resolved genomics methods for neuroscience discovery

B Bonev, CB Gonçalo, F Chen, S Codeluppi… - Nature …, 2024 - nature.com
Over the past decade, single-cell genomics technologies have allowed scalable profiling of
cell-type-specific features, which has substantially increased our ability to study cellular …

Single-cell RNA-seq methods to interrogate virus-host interactions

K Ratnasiri, AJ Wilk, MJ Lee, P Khatri… - Seminars in …, 2023 - Springer
The twenty-first century has seen the emergence of many epidemic and pandemic viruses,
with the most recent being the SARS-CoV-2-driven COVID-19 pandemic. As obligate …

Multiomic profiling of the acute stress response in the mouse hippocampus

LM von Ziegler, A Floriou-Servou, R Waag… - Nature …, 2022 - nature.com
The acute stress response mobilizes energy to meet situational demands and re-establish
homeostasis. However, the underlying molecular cascades are unclear. Here, we use a brief …

BIDCell: Biologically-informed self-supervised learning for segmentation of subcellular spatial transcriptomics data

X Fu, Y Lin, DM Lin, D Mechtersheimer, C Wang… - Nature …, 2024 - nature.com
Recent advances in subcellular imaging transcriptomics platforms have enabled high-
resolution spatial map** of gene expression, while also introducing significant analytical …