New insights into the degradation of synthetic pollutants in contaminated environments

P Bhatt, S Gangola, G Bhandari, W Zhang, D Maithani… - Chemosphere, 2021 - Elsevier
The environment is contaminated by synthetic contaminants owing to their extensive
applications globally. Hence, the removal of synthetic pollutants (SPs) from the environment …

Bacterial alkaline proteases: molecular approaches and industrial applications

R Gupta, Q Beg, P Lorenz - Applied microbiology and biotechnology, 2002 - Springer
Proteolytic enzymes are ubiquitous in occurrence, being found in all living organisms, and
are essential for cell growth and differentiation. The extracellular proteases are of …

Improving industrial yeast strains: exploiting natural and artificial diversity

J Steensels, T Snoek, E Meersman… - FEMS microbiology …, 2014 - academic.oup.com
Yeasts have been used for thousands of years to make fermented foods and beverages,
such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or …

[PDF][PDF] Design by directed evolution

FH Arnold - Accounts of chemical research, 1998 - cheme.caltech.edu
The stunning array of features and functions exhibited by proteins in nature should convince
most scientists of the power of evolutionary design processes. Natural selection acting on …

Molecular evolution by staggered extension process (StEP) in vitro recombination

H Zhao, L Giver, Z Shao, JA Affholter, FH Arnold - Nature biotechnology, 1998 - nature.com
We have developed a simple and efficient method for in vitro mutagenesis and
recombination of polynu-cleotide sequences. The staggered extension process (StEP) …

Lipases and their industrial applications: an overview

A Houde, A Kademi, D Leblanc - Applied biochemistry and biotechnology, 2004 - Springer
Abstract Lipases (triacylglycerol acylhydrolase, EC 3.1. 1.3) are part of the family of
hydrolases that act on carboxylic ester bonds. The physiologic role of lipases is to hydrolyze …

Biotechnology and bioremediation: successes and limitations

M Dua, A Singh, N Sethunathan, A Johri - Applied microbiology and …, 2002 - Springer
With advances in biotechnology, bioremediation has become one of the most rapidly
develo** fields of environmental restoration, utilizing microorganisms to reduce the …

Directed evolution of enzymes for industrial biocatalysis

JL Porter, RA Rusli, DL Ollis - ChemBioChem, 2016 - Wiley Online Library
Enzymes have the potential to catalyse a wide variety of chemical reactions. They are
increasingly being sought as environmentally friendly and cost‐effective alternatives to …

Metal-binding proteins and peptides in bioremediation and phytoremediation of heavy metals

M Mejáre, L Bülow - TRENDS in Biotechnology, 2001 - cell.com
The expression of metal-binding proteins or peptides in microorganisms and plants in order
to enhance heavy metal accumulation and/or tolerance has great potential. Several different …

Customizing lipases for biocatalysis: a survey of chemical, physical and molecular biological approaches

P Villeneuve, JM Muderhwa, J Graille… - Journal of molecular …, 2000 - Elsevier
Lipases (triacylglycerol ester hydrolases, EC 3.1. 1.3) are ubiquitous enzymes that catalyze
the breakdown of fats and oils with subsequent release of free fatty acids, diacylglycerols …