Environmental DNA metabarcoding: Transforming how we survey animal and plant communities

K Deiner, HM Bik, E Mächler, M Seymour… - Molecular …, 2017 - Wiley Online Library
The genomic revolution has fundamentally changed how we survey biodiversity on earth.
High‐throughput sequencing (“HTS”) platforms now enable the rapid sequencing of DNA …

[HTML][HTML] Environmental DNA–An emerging tool in conservation for monitoring past and present biodiversity

PF Thomsen, E Willerslev - Biological conservation, 2015 - Elsevier
The continuous decline in Earth's biodiversity represents a major crisis and challenge for the
21st century, and there is international political agreement to slow down or halt this decline …

Next‐generation monitoring of aquatic biodiversity using environmental DNA metabarcoding

A Valentini, P Taberlet, C Miaud, R Civade… - Molecular …, 2016 - Wiley Online Library
Global biodiversity in freshwater and the oceans is declining at high rates. Reliable tools for
assessing and monitoring aquatic biodiversity, especially for rare and secretive species, are …

Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects

KC Beng, RT Corlett - Biodiversity and conservation, 2020 - Springer
Conserving biodiversity in the face of ever-increasing human pressure is hampered by our
lack of basic information on species occurrence, distribution, abundance, habitat …

MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species

M Miya, Y Sato, T Fukunaga, T Sado… - Royal Society …, 2015 - royalsocietypublishing.org
We developed a set of universal PCR primers (MiFish-U/E) for metabarcoding
environmental DNA (eDNA) from fishes. Primers were designed using aligned whole …

The ecology of environmental DNA and implications for conservation genetics

MA Barnes, CR Turner - Conservation genetics, 2016 - Springer
Environmental DNA (eDNA) refers to the genetic material that can be extracted from bulk
environmental samples such as soil, water, and even air. The rapidly expanding study of …

Scrutinizing key steps for reliable metabarcoding of environmental samples

A Alberdi, O Aizpurua, MTP Gilbert… - Methods in Ecology …, 2018 - Wiley Online Library
Metabarcoding of environmental samples has many challenges and limitations that require
carefully considered laboratory and analysis workflows to ensure reliable results. We …

Can DNA-based ecosystem assessments quantify species abundance? Testing primer bias and biomass—sequence relationships with an innovative metabarcoding …

V Elbrecht, F Leese - PloS one, 2015 - journals.plos.org
Metabarcoding is an emerging genetic tool to rapidly assess biodiversity in ecosystems. It
involves high-throughput sequencing of a standard gene from an environmental sample and …

DNA metabarcoding—Need for robust experimental designs to draw sound ecological conclusions

L Zinger, A Bonin, IG Alsos, M Bálint, H Bik… - Molecular …, 2019 - Wiley Online Library
DNA metabarcoding, especially when coupled with high‐through‐put DNA sequencing, is
currently revolutionizing our capacity to assess biodiversity across a full range of taxa and …

Environmental DNA metabarcoding: A novel method for biodiversity monitoring of marine fish communities

M Miya - Annual Review of Marine Science, 2022 - annualreviews.org
Environmental DNA (eDNA) is genetic material that has been shed from macroorganisms. It
has received increased attention as an indirect marker for biodiversity monitoring. This …