Nanodiscs in membrane biochemistry and biophysics

IG Denisov, SG Sligar - Chemical reviews, 2017 - ACS Publications
Membrane proteins play a most important part in metabolism, signaling, cell motility,
transport, development, and many other biochemical and biophysical processes which …

The styrene–maleic acid copolymer: a versatile tool in membrane research

JM Dörr, S Scheidelaar, MC Koorengevel… - European Biophysics …, 2016 - Springer
A new and promising tool in membrane research is the detergent-free solubilization of
membrane proteins by styrene–maleic acid copolymers (SMAs). These amphipathic …

ATG9 vesicles comprise the seed membrane of mammalian autophagosomes

TJ Olivas, Y Wu, S Yu, L Luan, P Choi, ED Guinn… - Journal of Cell …, 2023 - rupress.org
As the autophagosome forms, its membrane surface area expands rapidly, while its volume
is kept low. Protein-mediated transfer of lipids from another organelle to the autophagosome …

A method for detergent-free isolation of membrane proteins in their local lipid environment

SC Lee, TJ Knowles, VLG Postis, M Jamshad… - Nature protocols, 2016 - nature.com
Despite the great importance of membrane proteins, structural and functional studies of
these proteins present major challenges. A significant hurdle is the extraction of the …

Solubilization of membrane proteins into functional lipid‐bilayer nanodiscs using a diisobutylene/maleic acid copolymer

AO Oluwole, B Danielczak, A Meister… - Angewandte Chemie …, 2017 - Wiley Online Library
Once removed from their natural environment, membrane proteins depend on membrane‐
mimetic systems to retain their native structures and functions. To this end, lipid‐bilayer …

Mass spectrometry methods for measuring protein stability

DD Vallejo, C Rojas Ramírez, KF Parson, Y Han… - Chemical …, 2022 - ACS Publications
Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …

Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: The power of native nanodiscs

JM Dörr, MC Koorengevel, M Schäfer… - Proceedings of the …, 2014 - pnas.org
A major obstacle in the study of membrane proteins is their solubilization in a stable and
active conformation when using detergents. Here, we explored a detergent-free approach to …

[HTML][HTML] Lipid membrane mimetics in functional and structural studies of integral membrane proteins

S Majeed, AB Ahmad, U Sehar, ER Georgieva - Membranes, 2021 - mdpi.com
Background: Open Access Editor's Choice Review Lipid Membrane Mimetics in Functional
and Structural Studies of Integral Membrane Proteins by Saman Majeed 1, Akram Bani …

An overview of the top ten detergents used for membrane protein crystallization

A Stetsenko, A Guskov - Crystals, 2017 - mdpi.com
To study integral membrane proteins, one has to extract them from the membrane—the step
that is typically achieved by the application of detergents. In this mini-review, we summarize …

Molecular model for the solubilization of membranes into nanodisks by styrene maleic acid copolymers

S Scheidelaar, MC Koorengevel, JD Pardo… - Biophysical journal, 2015 - cell.com
A recent discovery in membrane research is the ability of styrene-maleic acid (SMA)
copolymers to solubilize membranes in the form of nanodisks allowing extraction and …