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Nanodiscs in membrane biochemistry and biophysics
Membrane proteins play a most important part in metabolism, signaling, cell motility,
transport, development, and many other biochemical and biophysical processes which …
transport, development, and many other biochemical and biophysical processes which …
The styrene–maleic acid copolymer: a versatile tool in membrane research
JM Dörr, S Scheidelaar, MC Koorengevel… - European Biophysics …, 2016 - Springer
A new and promising tool in membrane research is the detergent-free solubilization of
membrane proteins by styrene–maleic acid copolymers (SMAs). These amphipathic …
membrane proteins by styrene–maleic acid copolymers (SMAs). These amphipathic …
ATG9 vesicles comprise the seed membrane of mammalian autophagosomes
As the autophagosome forms, its membrane surface area expands rapidly, while its volume
is kept low. Protein-mediated transfer of lipids from another organelle to the autophagosome …
is kept low. Protein-mediated transfer of lipids from another organelle to the autophagosome …
A method for detergent-free isolation of membrane proteins in their local lipid environment
Despite the great importance of membrane proteins, structural and functional studies of
these proteins present major challenges. A significant hurdle is the extraction of the …
these proteins present major challenges. A significant hurdle is the extraction of the …
Solubilization of membrane proteins into functional lipid‐bilayer nanodiscs using a diisobutylene/maleic acid copolymer
AO Oluwole, B Danielczak, A Meister… - Angewandte Chemie …, 2017 - Wiley Online Library
Once removed from their natural environment, membrane proteins depend on membrane‐
mimetic systems to retain their native structures and functions. To this end, lipid‐bilayer …
mimetic systems to retain their native structures and functions. To this end, lipid‐bilayer …
Mass spectrometry methods for measuring protein stability
Mass spectrometry is a central technology in the life sciences, providing our most
comprehensive account of the molecular inventory of the cell. In parallel with developments …
comprehensive account of the molecular inventory of the cell. In parallel with developments …
Detergent-free isolation, characterization, and functional reconstitution of a tetrameric K+ channel: The power of native nanodiscs
JM Dörr, MC Koorengevel, M Schäfer… - Proceedings of the …, 2014 - pnas.org
A major obstacle in the study of membrane proteins is their solubilization in a stable and
active conformation when using detergents. Here, we explored a detergent-free approach to …
active conformation when using detergents. Here, we explored a detergent-free approach to …
[HTML][HTML] Lipid membrane mimetics in functional and structural studies of integral membrane proteins
Background: Open Access Editor's Choice Review Lipid Membrane Mimetics in Functional
and Structural Studies of Integral Membrane Proteins by Saman Majeed 1, Akram Bani …
and Structural Studies of Integral Membrane Proteins by Saman Majeed 1, Akram Bani …
An overview of the top ten detergents used for membrane protein crystallization
To study integral membrane proteins, one has to extract them from the membrane—the step
that is typically achieved by the application of detergents. In this mini-review, we summarize …
that is typically achieved by the application of detergents. In this mini-review, we summarize …
Molecular model for the solubilization of membranes into nanodisks by styrene maleic acid copolymers
S Scheidelaar, MC Koorengevel, JD Pardo… - Biophysical journal, 2015 - cell.com
A recent discovery in membrane research is the ability of styrene-maleic acid (SMA)
copolymers to solubilize membranes in the form of nanodisks allowing extraction and …
copolymers to solubilize membranes in the form of nanodisks allowing extraction and …