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PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification
ABSTRACT PrimerBank (http://pga. mgh. harvard. edu/primerbank/) is a public resource for
the retrieval of human and mouse primer pairs for gene expression analysis by PCR and …
the retrieval of human and mouse primer pairs for gene expression analysis by PCR and …
Experiences with workflows for automating data-intensive bioinformatics
High-throughput technologies, such as next-generation sequencing, have turned molecular
biology into a data-intensive discipline, requiring bioinformaticians to use high-performance …
biology into a data-intensive discipline, requiring bioinformaticians to use high-performance …
Physico-chemical foundations underpinning microarray and next-generation sequencing experiments
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput
technologies such as microarrays and, in some cases, next-generation sequencing (NGS). A …
technologies such as microarrays and, in some cases, next-generation sequencing (NGS). A …
Characterization and improvement of RNA-Seq precision in quantitative transcript expression profiling
Motivation: Measurement precision determines the power of any analysis to reliably identify
significant signals, such as in screens for differential expression, independent of whether the …
significant signals, such as in screens for differential expression, independent of whether the …
A Comparative Analysis of Industrial Escherichia coli K–12 and B Strains in High-Glucose Batch Cultivations on Process-, Transcriptome- and Proteome Level
Escherichia coli K–12 and B strains are among the most frequently used bacterial hosts for
production of recombinant proteins on an industrial scale. To improve existing processes …
production of recombinant proteins on an industrial scale. To improve existing processes …
Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing
The pyrosequencing technology from 454 Life Sciences and a novel assembly approach for
cDNA sequences with the Newbler Assembler were used to achieve a major step forward to …
cDNA sequences with the Newbler Assembler were used to achieve a major step forward to …
Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays
Background DNA Microarrays are regarded as a valuable tool for basic and applied
research in microbiology. However, for many industrially important microorganisms the lack …
research in microbiology. However, for many industrially important microorganisms the lack …
FederatedCloudSim: a SLA-aware federated cloud simulation framework
Recent developments show that the standardization of cloud service descriptions and
exchange leads the way for the rise of cloud federations. In these federations CSPs (cloud …
exchange leads the way for the rise of cloud federations. In these federations CSPs (cloud …
The Gpr1-regulated Sur7 family protein Sfp2 is required for hyphal growth and cell wall stability in the mycoparasite Trichoderma atroviride
Mycoparasites, eg fungi feeding on other fungi, are prominent within the genus Trichoderma
and represent a promising alternative to chemical fungicides for plant disease control. We …
and represent a promising alternative to chemical fungicides for plant disease control. We …
[HTML][HTML] Transcriptomic changes in CHO cells after adaptation to suspension growth in protein-free medium analysed by a species-specific microarray
S Shridhar, G Klanert, N Auer, I Hernandez-Lopez… - Journal of …, 2017 - Elsevier
Abstract Chinese Hamster Ovary (CHO) cells are the preferred cell line for production of
biopharmaceuticals. These cells are capable to grow without serum supplementation, but …
biopharmaceuticals. These cells are capable to grow without serum supplementation, but …