PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification

A Spandidos, X Wang, H Wang… - Nucleic acids research, 2010 - academic.oup.com
ABSTRACT PrimerBank (http://pga. mgh. harvard. edu/primerbank/) is a public resource for
the retrieval of human and mouse primer pairs for gene expression analysis by PCR and …

Experiences with workflows for automating data-intensive bioinformatics

O Spjuth, E Bongcam-Rudloff, GC Hernández, L Forer… - Biology direct, 2015 - Springer
High-throughput technologies, such as next-generation sequencing, have turned molecular
biology into a data-intensive discipline, requiring bioinformaticians to use high-performance …

Physico-chemical foundations underpinning microarray and next-generation sequencing experiments

A Harrison, H Binder, A Buhot, CJ Burden… - Nucleic acids …, 2013 - academic.oup.com
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput
technologies such as microarrays and, in some cases, next-generation sequencing (NGS). A …

Characterization and improvement of RNA-Seq precision in quantitative transcript expression profiling

PP Łabaj, GG Leparc, BE Linggi, LM Markillie… - …, 2011 - academic.oup.com
Motivation: Measurement precision determines the power of any analysis to reliably identify
significant signals, such as in screens for differential expression, independent of whether the …

A Comparative Analysis of Industrial Escherichia coli K–12 and B Strains in High-Glucose Batch Cultivations on Process-, Transcriptome- and Proteome Level

K Marisch, K Bayer, T Scharl, J Mairhofer, PM Krempl… - PloS one, 2013 - journals.plos.org
Escherichia coli K–12 and B strains are among the most frequently used bacterial hosts for
production of recombinant proteins on an industrial scale. To improve existing processes …

Unraveling the Chinese hamster ovary cell line transcriptome by next-generation sequencing

J Becker, M Hackl, O Rupp, T Jakobi, J Schneider… - Journal of …, 2011 - Elsevier
The pyrosequencing technology from 454 Life Sciences and a novel assembly approach for
cDNA sequences with the Newbler Assembler were used to achieve a major step forward to …

Novel insights into the unfolded protein response using Pichia pastoris specific DNA microarrays

A Graf, B Gasser, M Dragosits, M Sauer, GG Leparc… - BMC genomics, 2008 - Springer
Background DNA Microarrays are regarded as a valuable tool for basic and applied
research in microbiology. However, for many industrially important microorganisms the lack …

FederatedCloudSim: a SLA-aware federated cloud simulation framework

A Kohne, M Spohr, L Nagel, O Spinczyk - Proceedings of the 2nd …, 2014 - dl.acm.org
Recent developments show that the standardization of cloud service descriptions and
exchange leads the way for the rise of cloud federations. In these federations CSPs (cloud …

The Gpr1-regulated Sur7 family protein Sfp2 is required for hyphal growth and cell wall stability in the mycoparasite Trichoderma atroviride

L Atanasova, S Gruber, A Lichius, T Radebner… - Scientific reports, 2018 - nature.com
Mycoparasites, eg fungi feeding on other fungi, are prominent within the genus Trichoderma
and represent a promising alternative to chemical fungicides for plant disease control. We …

[HTML][HTML] Transcriptomic changes in CHO cells after adaptation to suspension growth in protein-free medium analysed by a species-specific microarray

S Shridhar, G Klanert, N Auer, I Hernandez-Lopez… - Journal of …, 2017 - Elsevier
Abstract Chinese Hamster Ovary (CHO) cells are the preferred cell line for production of
biopharmaceuticals. These cells are capable to grow without serum supplementation, but …