Deciphering molecular interactions by proximity labeling

W Qin, KF Cho, PE Cavanagh, AY Ting - Nature methods, 2021 - nature.com
Many biological processes are executed and regulated through the molecular interactions of
proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the …

AP2/ERF transcription factors and their functions in Arabidopsis responses to abiotic stresses

K Wang, H Guo, Y Yin - Environmental and Experimental Botany, 2024 - Elsevier
Plants have to endure unfavorable environmental conditions due to their sessility. To adapt
to environmental constraints, plants have evolved intricate balancing mechanisms to …

Proximity labeling in mammalian cells with TurboID and split-TurboID

KF Cho, TC Branon, ND Udeshi, SA Myers, SA Carr… - Nature protocols, 2020 - nature.com
This protocol describes the use of TurboID and split-TurboID in proximity labeling
applications for map** protein–protein interactions and subcellular proteomes in live …

A non-canonical role of ATG8 in Golgi recovery from heat stress in plants

J Zhou, J Ma, C Yang, X Zhu, J Li, X Zheng, X Li… - Nature Plants, 2023 - nature.com
Above-optimal growth temperatures, usually referred to as heat stress (HS), pose a
challenge to organisms' survival as they interfere with essential physiological functions and …

Map** the signaling network of BIN2 kinase using TurboID-mediated biotin labeling and phosphoproteomics

TW Kim, CH Park, CC Hsu, YW Kim, YW Ko… - The Plant …, 2023 - academic.oup.com
Elucidating enzyme–substrate relationships in posttranslational modification (PTM) networks
is crucial for understanding signal transduction pathways but is technically difficult because …

BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition

H Zhang, C Rundle, N Winter, A Miricescu… - The Plant …, 2024 - academic.oup.com
Abstract BIG/DARK OVEREXPRESSION OF CAB1/TRANSPORT INHIBITOR RESPONSE3
is a 0.5 MDa protein associated with multiple functions in Arabidopsis (Arabidopsis thaliana) …

Proximity labeling in plants

SL Xu, R Shrestha, SS Karunadasa… - Annual review of plant …, 2023 - annualreviews.org
Proteins are workhorses in the cell; they form stable and more often dynamic, transient
protein–protein interactions, assemblies, and networks and have an intimate interplay with …

The development of proximity labeling technology and its applications in mammals, plants, and microorganisms

J Guo, S Guo, S Lu, J Gong, L Wang, L Ding… - Cell Communication and …, 2023 - Springer
Abstract Protein‒protein, protein‒RNA, and protein‒DNA interaction networks form the
basis of cellular regulation and signal transduction, making it crucial to explore these …

Proximity‐dependent labeling methods for proteomic profiling in living cells: An update

JA Bosch, CL Chen, N Perrimon - Wiley Interdisciplinary …, 2021 - Wiley Online Library
Characterizing the proteome composition of organelles and subcellular regions of living
cells can facilitate the understanding of cellular organization as well as protein interactome …

Guidelines for naming and studying plasma membrane domains in plants

Y Jaillais, E Bayer, DC Bergmann, MA Botella, Y Boutté… - Nature plants, 2024 - nature.com
Biological membranes play a crucial role in actively hosting, modulating and coordinating a
wide range of molecular events essential for cellular function. Membranes are organized …