Assembly of bacterial capsular polysaccharides and exopolysaccharides

C Whitfield, SS Wear, C Sande - Annual Review of Microbiology, 2020 - annualreviews.org
Polysaccharides are dominant features of most bacterial surfaces and are displayed in
different formats. Many bacteria produce abundant long-chain capsular polysaccharides …

Strategy to combat biofilms: a focus on biofilm dispersal enzymes

S Wang, Y Zhao, AP Breslawec, T Liang… - npj Biofilms and …, 2023 - nature.com
Bacterial biofilms, which consist of three-dimensional extracellular polymeric substance
(EPS), not only function as signaling networks, provide nutritional support, and facilitate …

[HTML][HTML] Structural diversity of carbohydrate esterases

AM Nakamura, AS Nascimento, I Polikarpov - Biotechnology Research and …, 2017 - Elsevier
Carbohydrate esterases (CEs) catalyze the de-O or de-N-acylation by removing the ester
decorations from carbohydrates. CEs are currently classified in 15 families in the …

Enzymatic modifications of exopolysaccharides enhance bacterial persistence

GB Whitfield, LS Marmont, PL Howell - Frontiers in microbiology, 2015 - frontiersin.org
Biofilms are surface-attached communities of bacterial cells embedded in a self-produced
matrix that are found ubiquitously in nature. The biofilm matrix is composed of various …

Identification of poly-N-acetylglucosamine as a major polysaccharide component of the Bacillus subtilis biofilm matrix

D Roux, C Cywes-Bentley, YF Zhang, S Pons… - Journal of Biological …, 2015 - ASBMB
Bacillus subtilis is intensively studied as a model organism for the development of bacterial
biofilms or pellicles. A key component is currently undefined exopolysaccharides produced …

Deacetylation of fungal exopolysaccharide mediates adhesion and biofilm formation

MJ Lee, AM Geller, NC Bamford, H Liu, FN Gravelat… - MBio, 2016 - Am Soc Microbiol
The mold Aspergillus fumigatus causes invasive infection in immunocompromised patients.
Recently, galactosaminogalactan (GAG), an exopolysaccharide composed of galactose and …

Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formation

NC Bamford, F Le Mauff, JC Van Loon… - Nature …, 2020 - nature.com
The exopolysaccharide galactosaminogalactan (GAG) is an important virulence factor of the
fungal pathogen Aspergillus fumigatus. Deletion of a gene encoding a putative deacetylase …

Diverse mechanisms of polysaccharide biosynthesis, assembly and secretion across kingdoms

Z Stephens, LFL Wilson, J Zimmer - Current Opinion in Structural Biology, 2023 - Elsevier
Polysaccharides are essential biopolymers produced in all kingdoms of life. On the cell
surface, they represent versatile architectural components, forming protective capsules and …

Substrate recognition and specificity of chitin deacetylases and related family 4 carbohydrate esterases

H Aragunde, X Biarnés, A Planas - International journal of molecular …, 2018 - mdpi.com
Carbohydrate esterases family 4 (CE4 enzymes) includes chitin and peptidoglycan
deacetylases, acetylxylan esterases, and poly-N-acetylglucosamine deacetylases that act …

A systematic pipeline for classifying bacterial operons reveals the evolutionary landscape of biofilm machineries

C Bundalovic-Torma, GB Whitfield… - PLoS computational …, 2020 - journals.plos.org
In bacteria functionally related genes comprising metabolic pathways and protein
complexes are frequently encoded in operons and are widely conserved across …