A lack of generic and effective genetic manipulation methods for Pseudomonas has
restricted fundamental research and utilization of this genus for biotechnology applications …

Recombineering is a valuable technique for generating recombinant DNA in vivo, primarily
in bacterial cells, and is based on homologous recombination using phage-encoded …

The technology of recombineering, in vivo genetic engineering, was initially developed in
Escherichia coli and uses bacteriophage‐encoded homologous recombination proteins to …

Bacterial secondary metabolites represent an invaluable source of bioactive molecules for
the pharmaceutical and agrochemical industries. Although screening campaigns for the …

Introduction Transcription of biological nitrogen fixation (nif) genes is activated by the NifA
protein which recognizes specific activating sequences upstream of σ 54-dependent nif …

Phage-encoded homologous recombination (PEHR) is an efficient tool for bacterial genome
editing. We previously developed and utilized a Pseudomonas-specific PEHR system …

The ability of bacteria to use natural carbon sources greatly affects their growth and survival
in the environment. Bacteria have evolved versatile abilities to use environmental carbon …

Sinorhizobium meliloti Rm1021 (S. meliloti Rm1021) is a Gram-negative, soil-dwelling α-
proteobacterium which serves as a model microorganism for the studies of symbiotic …