[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …
molecules have led to substantial improvements in accuracy, read length and throughput …
Coming of age: ten years of next-generation sequencing technologies
Since the completion of the human genome project in 2003, extraordinary progress has
been made in genome sequencing technologies, which has led to a decreased cost per …
been made in genome sequencing technologies, which has led to a decreased cost per …
Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation
Long-read single-molecule sequencing has revolutionized de novo genome assembly and
enabled the automated reconstruction of reference-quality genomes. However, given the …
enabled the automated reconstruction of reference-quality genomes. However, given the …
The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community
Nanopore DNA strand sequencing has emerged as a competitive, portable technology.
Reads exceeding 150 kilobases have been achieved, as have in-field detection and …
Reads exceeding 150 kilobases have been achieved, as have in-field detection and …
A complete bacterial genome assembled de novo using only nanopore sequencing data
We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-
Mb contig using only nanopore data. Our method has three stages:(i) overlaps are detected …
Mb contig using only nanopore data. Our method has three stages:(i) overlaps are detected …
Oxford Nanopore MinION sequencing and genome assembly
H Lu, F Giordano, Z Ning - Genomics, Proteomics and …, 2016 - academic.oup.com
The revolution of genome sequencing is continuing after the successful second-generation
sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by …
sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by …
From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy
Nanopore sequencing is a rapidly maturing technology delivering long reads in real time on
a portable instrument at low cost. Not surprisingly, the community has rapidly taken up this …
a portable instrument at low cost. Not surprisingly, the community has rapidly taken up this …
Towards precision medicine
EA Ashley - Nature Reviews Genetics, 2016 - nature.com
There is great potential for genome sequencing to enhance patient care through improved
diagnostic sensitivity and more precise therapeutic targeting. To maximize this potential …
diagnostic sensitivity and more precise therapeutic targeting. To maximize this potential …
Third-generation sequencing: the spearhead towards the radical transformation of modern genomics
Although next-generation sequencing (NGS) technology revolutionized sequencing, offering
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …
Piercing the dark matter: bioinformatics of long-range sequencing and map**
Several new genomics technologies have become available that offer long-read sequencing
or long-range map** with higher throughput and higher resolution analysis than ever …
or long-range map** with higher throughput and higher resolution analysis than ever …