[HTML][HTML] Nanopore sequencing technology, bioinformatics and applications

Y Wang, Y Zhao, A Bollas, Y Wang, KF Au - Nature biotechnology, 2021 - nature.com
Rapid advances in nanopore technologies for sequencing single long DNA and RNA
molecules have led to substantial improvements in accuracy, read length and throughput …

Coming of age: ten years of next-generation sequencing technologies

S Goodwin, JD McPherson, WR McCombie - Nature reviews genetics, 2016 - nature.com
Since the completion of the human genome project in 2003, extraordinary progress has
been made in genome sequencing technologies, which has led to a decreased cost per …

Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation

S Koren, BP Walenz, K Berlin, JR Miller… - Genome …, 2017 - genome.cshlp.org
Long-read single-molecule sequencing has revolutionized de novo genome assembly and
enabled the automated reconstruction of reference-quality genomes. However, given the …

The Oxford Nanopore MinION: delivery of nanopore sequencing to the genomics community

M Jain, HE Olsen, B Paten, M Akeson - Genome biology, 2016 - Springer
Nanopore DNA strand sequencing has emerged as a competitive, portable technology.
Reads exceeding 150 kilobases have been achieved, as have in-field detection and …

A complete bacterial genome assembled de novo using only nanopore sequencing data

NJ Loman, J Quick, JT Simpson - Nature methods, 2015 - nature.com
We have assembled de novo the Escherichia coli K-12 MG1655 chromosome in a single 4.6-
Mb contig using only nanopore data. Our method has three stages:(i) overlaps are detected …

Oxford Nanopore MinION sequencing and genome assembly

H Lu, F Giordano, Z Ning - Genomics, Proteomics and …, 2016 - academic.oup.com
The revolution of genome sequencing is continuing after the successful second-generation
sequencing (SGS) technology. The third-generation sequencing (TGS) technology, led by …

From squiggle to basepair: computational approaches for improving nanopore sequencing read accuracy

FJ Rang, WP Kloosterman, J de Ridder - Genome biology, 2018 - Springer
Nanopore sequencing is a rapidly maturing technology delivering long reads in real time on
a portable instrument at low cost. Not surprisingly, the community has rapidly taken up this …

Towards precision medicine

EA Ashley - Nature Reviews Genetics, 2016 - nature.com
There is great potential for genome sequencing to enhance patient care through improved
diagnostic sensitivity and more precise therapeutic targeting. To maximize this potential …

Third-generation sequencing: the spearhead towards the radical transformation of modern genomics

K Athanasopoulou, MA Boti, PG Adamopoulos… - Life, 2021 - mdpi.com
Although next-generation sequencing (NGS) technology revolutionized sequencing, offering
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …

Piercing the dark matter: bioinformatics of long-range sequencing and map**

FJ Sedlazeck, H Lee, CA Darby, MC Schatz - Nature Reviews Genetics, 2018 - nature.com
Several new genomics technologies have become available that offer long-read sequencing
or long-range map** with higher throughput and higher resolution analysis than ever …