Good practices and recommendations for using and benchmarking computational metabolomics metabolite annotation tools

NF de Jonge, K Mildau, D Meijer, JJR Louwen… - Metabolomics, 2022 - Springer
Background Untargeted metabolomics approaches based on mass spectrometry obtain
comprehensive profiles of complex biological samples. However, on average only 10% of …

Regulatory T cells shield muscle mitochondria from interferon-γ–mediated damage to promote the beneficial effects of exercise

PK Langston, Y Sun, BA Ryback, AL Mueller… - Science …, 2023 - science.org
Exercise enhances physical performance and reduces the risk of many disorders such as
cardiovascular disease, type 2 diabetes, dementia, and cancer. Exercise characteristically …

Spec2Vec: Improved mass spectral similarity scoring through learning of structural relationships

F Huber, L Ridder, S Verhoeven… - PLoS computational …, 2021 - journals.plos.org
Spectral similarity is used as a proxy for structural similarity in many tandem mass
spectrometry (MS/MS) based metabolomics analyses such as library matching and …

MS2DeepScore: a novel deep learning similarity measure to compare tandem mass spectra

F Huber, S van der Burg, JJJ van der Hooft… - Journal of …, 2021 - Springer
Mass spectrometry data is one of the key sources of information in many workflows in
medicine and across the life sciences. Mass fragmentation spectra are generally considered …

Navigating common pitfalls in metabolite identification and metabolomics bioinformatics

EM Novoa-del-Toro, M Witting - Metabolomics, 2024 - Springer
Background Metabolomics, the systematic analysis of small molecules in a given biological
system, emerged as a powerful tool for different research questions. Newer, better, and …

Flash entropy search to query all mass spectral libraries in real time

Y Li, O Fiehn - Nature Methods, 2023 - nature.com
Public repositories of metabolomics mass spectra encompass more than 1 billion entries.
With open search, dot product or entropy similarity, comparisons of a single tandem mass …

Annotating metabolite mass spectra with domain-inspired chemical formula transformers

S Goldman, J Wohlwend, M Stražar… - Nature Machine …, 2023 - nature.com
Metabolomics studies have identified small molecules that mediate cell signaling,
competition and disease pathology, in part due to large-scale community efforts to measure …

Evolutionary metabolomics of specialized metabolism diversification in the genus Nicotiana highlights N-acylnornicotine innovations

D Elser, D Pflieger, C Villette, B Moegle, L Miesch… - Science …, 2023 - science.org
Specialized metabolite (SM) diversification is a core process to plants' adaptation to diverse
ecological niches. Here, we implemented a computational mass spectrometry–based …

MS2Query: reliable and scalable MS2 mass spectra-based analogue search

NF de Jonge, JJR Louwen, E Chekmeneva… - Nature …, 2023 - nature.com
Metabolomics-driven discoveries of biological samples remain hampered by the grand
challenge of metabolite annotation and identification. Only few metabolites have an …

Comparison of cosine, modified cosine, and neutral loss based spectrum alignment for discovery of structurally related molecules

W Bittremieux, R Schmid, F Huber… - Journal of the …, 2022 - ACS Publications
Spectrum alignment of tandem mass spectrometry (MS/MS) data using the modified cosine
similarity and subsequent visualization as molecular networks have been demonstrated to …