The ability to engineer biological systems, whether to introduce novel functionality or
improved performance, is a cornerstone of biotechnology and synthetic biology. Typically …

Directed evolution is a powerful approach to study the molecular basis of protein evolution
and to engineer proteins for a wide range of applications in synthetic organic chemistry and …

With advances in machine learning (ML)-assisted protein engineering, models based on
data, biophysics, and natural evolution are being used to propose informed libraries of …

Motivation Scientists increasingly rely on intelligent information systems to help them in their
daily tasks, in particular for managing research objects, like publications or datasets. The …

CodonGenie, freely available from http://codon. synbiochem. co. uk, is a simple web
application for designing ambiguous codons to support protein mutagenesis applications …

Drug resistance is a major healthcare challenge, resulting in a continuous need to develop
new inhibitors. The development of these inhibitors requires an understanding of the …

Saturation mutagenesis is a semi-rational approach for protein engineering where sites are
saturated either entirely or partially to include amino acids of interest. We previously …

Saturation mutagenesis is conveniently located between the two extremes of protein
engineering, namely random mutagenesis, and rational design. It involves mutating a …

To understand protein structure emergence is to comprehend the evolutionary transition
from messy chemistry to the first heritable molecular systems. Early proteins were probably …

I present the utility of unique DNA barcodes to tag distinct genotypes and subsequently link
them to phenotypes. Such molecular tagging allowed us to perform multiplexed phenotype …