Assisted reproductive techniques developed for cattle in the last 25 years, like ovum pick up
(OPU), intracytoplasmic sperm injection (ICSI), and somatic cell nuclear transfer, have been …

Mammalian oocytes can reprogram differentiated somatic cells into a totipotent state through
somatic cell nuclear transfer (SCNT), which is known as cloning. Although many mammalian …

Cloning animals by somatic cell nuclear transfer (SCNT) has remained an uncontrollable
process for many years. High rates of embryonic losses, stillbirths, and postnatal mortality …

Nuclear transfer is a complex multistep procedure that includes oocyte maturation, cell cycle
synchronization of donor cells, enucleation, cell fusion, oocyte activation and embryo …

The rapid decline in fertility that has been occurring to high-producing dairy cows in the past
50 years seems to be associated with metabolic disturbances such as ketosis, supporting …

Epigenetic mechanisms allow the establishment and maintenance of multiple cellular
phenotypes from a single genomic code. At the initiation of development, the oocyte and …

The nucleus of a differentiated cell can be reprogrammed to a totipotent state by exposure to
the cytoplasm of an enucleated oocyte, and the reconstructed nuclear transfer embryo can …

It remained unknown whether HDAC6 affected the histone deacetylation of in vitro
maturation oocytes and the reprogramming of nuclear transplantation in pig. Our results …

Cloning of mammals by somatic cell nuclear transfer (SCNT) is still plagued by low
efficiency. The epigenetic modifications established during cellular differentiation are a …

The birth of Dolly through somatic cell nuclear transfer (SCNT) was a major scientific
breakthrough of the last century. Yet, while significant progress has been achieved across …