The third edition of Flow Cytometry Guidelines provides the key aspects to consider when
performing flow cytometry experiments and includes comprehensive sections describing …

A small population of B cells exists in lymphoid tissues and body cavities of mice that is
distinct in development, phenotype, and function from the majority (B-2) B cell population …

These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

The transcriptional factor NF-κB is a nuclear factor involved in both physiological and
pathological processes. This factor can control the transcription of more than 400 genes …

Innate-like B-1a cells provide a first line of defense against pathogens, yet little is known
about their transcriptional control. Here we identified an essential role for the transcription …

B-1a cells are long-lived, self-renewing innate-like B cells that predominantly inhabit the
peritoneal and pleural cavities. In contrast to conventional B-2 cells, B-1a cells have a …

Traditional models of lymphopoiesis present B and T cell development as a linear process
that initiates in the fetus and continues after birth in the bone marrow and thymus …

Natural IgM plays a critical role in protection from pathogens and the prevention of
autoimmunity. While its importance has been shown in many different settings, its origins are …

The gastrointestinal (GI) tract must balance the extraction of energy and metabolic end-
products from ingested nutrition and resident gut microbes and the maintenance of a …

Highlights•The BCR specificity plays an instructive role in the B-1a cell lineage choice.•The
clonal composition of B-1a cells is shaped throughout the lifetime of a mouse.•Premature Igk …