RNA processing plays a central role in accurately transmitting genetic information into
functional RNA and protein regulators. To fully appreciate the RNA life-cycle, tools to …

Cellular responses to TNF are inherently heterogeneous within an isogenic cell population
and across different cell types. TNF promotes cell survival by activating pro-inflammatory NF …

Summary The lncRNA **st forms∼ 50 diffraction-limited foci to transcriptionally silence one
X chromosome. How this small number of RNA foci and interacting proteins regulate a much …

Signals in many biological processes can be amplified by recruiting multiple copies of
regulatory proteins to a site of action. Harnessing this principle, we have developed a …

Biomolecular condensates, often assembled through phase transition mechanisms, play key
roles in organizing diverse cellular activities. The material properties of condensates …

Abstract Prokaryotic type II CRISPR-Cas systems can be adapted to enable targeted
genome modifications across a range of eukaryotes,,,,,,. Here we engineer this system to …

As our understanding of the cell interior has grown, we have come to appreciate that most
cellular operations are localized, that is, they occur at discrete and identifiable locations or …

RNA-guided Cas9 nucleases derived from clustered regularly interspaced short palindromic
repeats (CRISPR)-Cas systems have dramatically transformed our ability to edit the …

The spatial regulation of protein translation is an efficient way to create functional and
structural asymmetries in cells. Recent research has furthered our understanding of how …

Translation is under tight spatial and temporal controls to ensure protein production in the
right time and place in cells. Methods that allow real-time, high-resolution visualization of …