Application and challenge of 3rd generation sequencing for clinical bacterial studies

M Ben Khedher, K Ghedira, JM Rolain… - International Journal of …, 2022 - mdpi.com
Over the past 25 years, the powerful combination of genome sequencing and bioinformatics
analysis has played a crucial role in interpreting information encoded in bacterial genomes …

Transcriptome assembly from long-read RNA-seq alignments with StringTie2

S Kovaka, AV Zimin, GM Pertea, R Razaghi… - Genome biology, 2019 - Springer
RNA sequencing using the latest single-molecule sequencing instruments produces reads
that are thousands of nucleotides long. The ability to assemble these long reads can greatly …

Intron retention and its impact on gene expression and protein diversity: A review and a practical guide

DF Grabski, L Broseus, B Kumari… - Wiley …, 2021 - Wiley Online Library
Intron retention (IR) occurs when a complete and unspliced intron remains in mature mRNA.
An increasing body of literature has demonstrated a major role for IR in numerous biological …

Flow decomposition with subpath constraints

L Williams, AI Tomescu… - IEEE/ACM Transactions on …, 2022 - ieeexplore.ieee.org
Flow network decomposition is a natural model for problems where we are given a flow
network arising from superimposing a set of weighted paths and would like to recover the …

Using metatranscriptomics to estimate the diversity and composition of zooplankton communities

MLD Lopez, Y Lin, M Sato, C Hsieh… - Molecular Ecology …, 2022 - Wiley Online Library
DNA metabarcoding is a rapid, high‐resolution tool used for biomonitoring complex
zooplankton communities. However, diversity estimates derived with this approach can be …

Safety in multi-assembly via paths appearing in all path covers of a DAG

M Cáceres, B Mumey, E Husić, R Rizzi… - IEEE/ACM …, 2021 - ieeexplore.ieee.org
A multi-assembly problem asks to reconstruct multiple genomic sequences from mixed
reads sequenced from all of them. Standard formulations of such problems model a solution …

Using minimum path cover to boost dynamic programming on DAGs: co-linear chaining extended

A Kuosmanen, T Paavilainen, T Gagie, R Chikhi… - … Molecular Biology: 22nd …, 2018 - Springer
Aligning sequencing reads on graph representations of genomes is an important ingredient
of pan-genomics. Such approaches typically find a set of local anchors that indicate …

TALC: transcript-level aware long-read correction

L Broseus, A Thomas, AJ Oldfield, D Severac… - …, 2020 - academic.oup.com
Motivation Long-read sequencing technologies are invaluable for determining complex RNA
transcript architectures but are error-prone. Numerous 'hybrid correction'algorithms have …

CStone: A de novo transcriptome assembler for short-read data that identifies non-chimeric contigs based on underlying graph structure

R Linheiro, J Archer - PLoS computational biology, 2021 - journals.plos.org
With the exponential growth of sequence information stored over the last decade, including
that of de novo assembled contigs from RNA-Seq experiments, quantification of chimeric …

A novel model based on immune-related genes for differentiating biliary atresia from other cholestatic diseases

T Li, Q Zheng, R Zhang, S Liu, Y Lin, J Zhan - Pediatric Surgery …, 2022 - Springer
Purpose Based on a public gene expression database, this study established the immune-
related genetic model that distinguished BA from other cholestasis diseases (DC) for the first …