Immunoglobulin E (IgE) antibodies are well known for their role in mediating allergic
reactions, and their powerful effector functions activated through binding to Fc receptors …

Amongst the most important outputs of the biopharmaceutical industry are recombinant
proteins, many of which are produced by integrating transgenes into the genomes of …

A single-chain fragment variable (scFv) recognizing β2-glycoprotein 1 (β2GPI) from humans
and other species was isolated from a human phage display library and engineered to …

The isolation of stably transfected cell lines suitable for the manufacture of biotherapeutic
protein products can be an arduous process relying on the identification of a high …

The efficacy of antibody-based immunotherapy is due to the activation of apoptosis, the
engagement of antibody-dependent cellular cytotoxicity and complement-dependent …

Abstract Ubiquitous Chromatin-opening Elements (UCOEs) are defined by their ability to
consistently confer stable, site of integration-independent transgene expression that is …

Highlights•UCOE-containing cells show advantage in recombinant antibody
expression.•Cell line generation involving the use of semi-solid cloning and ClonePix …

Osteopontin is a proinflammatory cytokine and plays a pathogenetic role in multiple
sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE), by …

Mammalian cells are the preferred choice system for the production of complex molecules,
such as recombinant therapeutic proteins. Although the technology for increasing the yield …

Whenever the function of a recombinant protein depends on post-translational processing,
mammalian cells become an indispensable tool for their production. This is particularly true …