High-resolution native mass spectrometry

S Tamara, MA den Boer, AJR Heck - Chemical Reviews, 2021 - ACS Publications
Native mass spectrometry (MS) involves the analysis and characterization of
macromolecules, predominantly intact proteins and protein complexes, whereby as much as …

Ultraviolet photodissociation mass spectrometry for analysis of biological molecules

JS Brodbelt, LJ Morrison, I Santos - Chemical reviews, 2019 - ACS Publications
The development of new ion-activation/dissociation methods continues to be one of the most
active areas of mass spectrometry owing to the broad applications of tandem mass …

Protein–small molecule interactions in native mass spectrometry

JL Bennett, GTH Nguyen, WA Donald - Chemical Reviews, 2021 - ACS Publications
Small molecule drug discovery has been propelled by the continual development of novel
scientific methodologies to occasion therapeutic advances. Although established …

An integrated native mass spectrometry and top-down proteomics method that connects sequence to structure and function of macromolecular complexes

H Li, HH Nguyen, RR Ogorzalek Loo… - Nature …, 2018 - nature.com
Mass spectrometry (MS) has become a crucial technique for the analysis of protein
complexes. Native MS has traditionally examined protein subunit arrangements, while …

Top-down proteomics: ready for prime time?

B Chen, KA Brown, Z Lin, Y Ge - Analytical chemistry, 2017 - ACS Publications
Beyond the genetic code, the human proteome is greatly diversified by genetic variations,
alternative splicing of RNA transcripts, and post-translational modifications (PTMs). 6, 7 In …

Ion activation methods for peptides and proteins

JS Brodbelt - Analytical chemistry, 2016 - ACS Publications
The analysis of peptides and proteins as well as the grander scope of proteomics (large
scale study of proteins) has been advanced by the development of a versatile array of ion …

Top or middle? Up or down? Toward a standard lexicon for protein top-down and allied mass spectrometry approaches

F Lermyte, YO Tsybin, PB O'Connor… - Journal of the American …, 2019 - ACS Publications
In recent years, there has been increasing interest in top-down mass spectrometry (TDMS)
approaches for protein analysis, driven both by technological advancements and efforts …

The diverse and expanding role of mass spectrometry in structural and molecular biology

P Lössl, M van de Waterbeemd, AJR Heck - The EMBO journal, 2016 - embopress.org
The emergence of proteomics has led to major technological advances in mass
spectrometry (MS). These advancements not only benefitted MS‐based high‐throughput …

Mass spectrometry of protein complexes: from origins to applications

S Mehmood, TM Allison… - Annual Review of Physical …, 2015 - annualreviews.org
Now routine is the ability to investigate soluble and membrane protein complexes in the gas
phase of a mass spectrometer while preserving folded structure and ligand-binding …

Approaches to heterogeneity in native mass spectrometry

AD Rolland, JS Prell - Chemical reviews, 2021 - ACS Publications
Native mass spectrometry (MS) is aimed at preserving and determining the native structure,
composition, and stoichiometry of biomolecules and their complexes from solution after they …