Fluorescence resonance energy transfer (FRET) is a powerful spectroscopic method for
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …

Many proteins contain large structurally disordered regions or are entirely disordered under
physiological conditions. The functions of these intrinsically disordered proteins (IDPs) often …

Single-molecule Förster-resonance energy transfer (smFRET) experiments allow the study
of biomolecular structure and dynamics in vitro and in vivo. We performed an international …

Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to
determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However …

When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Cas12a, also known as Cpf1, is a type VA CRISPR-Cas RNA-guided endonuclease that is
used for genome editing based on its ability to generate specific dsDNA breaks. Here, we …

Inorganic polyphosphate (polyP) is an ancient energy metabolite and phosphate store that
occurs ubiquitously in all organisms. The vacuolar transporter chaperone (VTC) complex …

Ever since their introduction two decades ago, single-molecule (SM) fluorescence methods
have matured and branched out to address numerous biological questions, which were …

A complete understanding of a protein-folding mechanism requires description of the
distribution of microscopic pathways that connect the folded and unfolded states. This …

In this article, we demonstrate the new method of pulsed interleaved excitation (PIE), which
can be used to extend the capabilities of multiple-color fluorescence imaging, fluorescence …