Structural biologists have traditionally approached cellular complexity in a reductionist
manner in which the cellular molecular components are fractionated and purified before …

▪ Abstract Electron tomography (ET) is uniquely suited to obtain three-dimensional
reconstructions of pleomorphic structures, such as cells, organelles or supramolecular …

A new method was developed to acquire images automatically at a series of specimen tilts,
as required for tomographic reconstruction. The method uses changes in specimen position …

We report here on the current state of our efforts in automated molecular microscopy. Our
primary automated data acquisition software system, Leginon, has been completely …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

Electron tomography of vitrified cells is a noninvasive three-dimensional imaging technique
that opens up new vistas for exploring the supramolecular organization of the cytoplasm. We …

Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …

Cryo-electron tomography (cryo-ET) allows 3D volumes to be reconstructed from a set of 2D
projection images of a tilted biological sample. It allows densities to be resolved in 3D that …

1. Introduction 3082. Electron microscopy 3112.1 Specimen preparation 3112.2 The
electron microscope 3112.3 Acceleration voltage, defocus, and the electron gun 3122.4 …

The chapter about the Gram-positive bacterial cell wall gives a brief historical background
on the discovery of Gram-positive cell walls and their constituents and microscopic methods …