Extracellular vesicles (EVs) are particles naturally released from cells that are delimited by a
lipid bilayer and are unable to replicate. How the EVs cross the Blood–Brain barrier (BBB) in …

We review the emerging method of super-resolved cryogenic correlative light and electron
microscopy (srCryoCLEM). Super-resolution (SR) fluorescence microscopy and cryogenic …

Cryo-electron tomography (cryo-ET) has become a powerful approach to study the high-
resolution structure of cellular macromolecular machines in situ. However, the current …

Cryo-electron tomography (cryo-ET) is a revolutionary technique for resolving the structure
of subcellular organelles and macromolecular complexes in their cellular context. However …

Cryo-correlative light and electron microscopy (CLEM) is a technique that uses the
spatiotemporal cues from fluorescence light microscopy (FLM) to investigate the high …

Correlative light and electron microscopy (CLEM) pipelines serve to integrate the imaging
modalities of fluorescence light microscopy (FLM) and cryogenic electron microscopy (cryo …

Membrane modulation is a key part of cellular life. Critical to processes like energy
production, cell division, trafficking, migration and even pathogen entry, defects in …

Correlative light and electron microscopy (CLEM) combines the strengths of both light and
electron imaging modalities and enables linking of biological spatiotemporal information …

Cryo-focused ion beam (cryo-FIB) milling technology has been developed for the fabrication
of cryo-lamella of frozen native specimens for study by in situ cryo-electron tomography (cryo …

Electron microscopy (EM) reveals cellular ultrastructure at high definition but faces the
challenges of identification of specific subcellular structures and localization of specific …