Single-vesicle assays using liposomes and cell-derived vesicles: from modeling complex membrane processes to synthetic biology and biomedical applications

H Pick, AC Alves, H Vogel - Chemical reviews, 2018 - ACS Publications
The plasma membrane is of central importance for defining the closed volume of cells in
contradistinction to the extracellular environment. The plasma membrane not only serves as …

Luminescence lifetime imaging of three-dimensional biological objects

RI Dmitriev, X Intes, MM Barroso - Journal of Cell Science, 2021 - journals.biologists.com
ABSTRACT A major focus of current biological studies is to fill the knowledge gaps between
cell, tissue and organism scales. To this end, a wide array of contemporary optical analytical …

Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane

M Amaro, F Reina, M Hof, C Eggeling… - Journal of physics D …, 2017 - iopscience.iop.org
Lipid packing is a crucial feature of cellular membranes. Quantitative analysis of membrane
lipid packing can be achieved using polarity sensitive probes whose emission spectrum …

A Rotational BODIPY Nucleotide: An Environment‐Sensitive Fluorescence‐Lifetime Probe for DNA Interactions and Applications in Live‐Cell Microscopy

D Dziuba, P Jurkiewicz, M Cebecauer… - Angewandte …, 2016 - Wiley Online Library
Fluorescent probes for detecting the physical properties of cellular structures have become
valuable tools in life sciences. The fluorescence lifetime of molecular rotors can be used to …

Laurdan discerns lipid membrane hydration and cholesterol content

H Orlikowska-Rzeznik, E Krok… - The Journal of …, 2023 - ACS Publications
Studies of biological membrane heterogeneity particularly benefit from the use of the
environment-sensitive fluorescent probe Laurdan, for which shifts in the emission, produced …

With or without rafts? Alternative views on cell membranes

E Sevcsik, GJ Schütz - Bioessays, 2016 - Wiley Online Library
The fundamental mechanisms of protein and lipid organization at the plasma membrane
have continued to engage researchers for decades. Among proposed models, one idea has …

GM1 Ganglioside Inhibits β‐Amyloid Oligomerization Induced by Sphingomyelin

M Amaro, R Šachl, G Aydogan… - Angewandte Chemie …, 2016 - Wiley Online Library
Abstract β‐Amyloid (Aβ) oligomers are neurotoxic and implicated in Alzheimer's disease.
Neuronal plasma membranes may mediate formation of Aβ oligomers in vivo. Membrane …

Graphene oxide-based fluorescent biosensors for pathogenic bacteria detection: A review

S Liu, F Lu, S Chen, Y Ning - Analytica Chimica Acta, 2024 - Elsevier
Background Pathogenic bacteria are widespread in nature and can cause infections and
various complications, thereby posing a severe risk to public health. Therefore, simple …

Cholesterol under oxidative stress—How lipid membranes sense oxidation as cholesterol is being replaced by oxysterols

W Kulig, A Olżyńska, P Jurkiewicz, AM Kantola… - Free radical biology and …, 2015 - Elsevier
The behavior of oxysterols in phospholipid membranes and their effects on membrane
properties were investigated by means of dynamic light scattering, fluorescence …

A unifying framework for amyloid-mediated membrane damage: The lipid-chaperone hypothesis

C Tempra, F Scollo, M Pannuzzo, F Lolicato… - … et Biophysica Acta (BBA …, 2022 - Elsevier
Over the past thirty years, researchers have highlighted the role played by a class of proteins
or polypeptides that forms pathogenic amyloid aggregates in vivo, including i) the amyloid …