Bioinformatic challenges for DNA metabarcoding of plants and animals
Almost all empirical studies in ecology have to identify the species involved in the ecological
process under examination. DNA metabarcoding, which couples the principles of DNA …
process under examination. DNA metabarcoding, which couples the principles of DNA …
A genomic perspective on the shortcomings of mitochondrial DNA for “barcoding” identification
Approximately 600-bp sequences of mitochondrial DNA (mtDNA) have been designated as
“DNA barcodes” and have become one of the most contentious and animated issues in the …
“DNA barcodes” and have become one of the most contentious and animated issues in the …
Power and limitations of the chloroplast trn L (UAA) intron for plant DNA barcoding
DNA barcoding should provide rapid, accurate and automatable species identifications by
using a standardized DNA region as a tag. Based on sequences available in GenBank and …
using a standardized DNA region as a tag. Based on sequences available in GenBank and …
Environmental metabarcodes for insects: in silico PCR reveals potential for taxonomic bias
Studies of insect assemblages are suited to the simultaneous DNA‐based identification of
multiple taxa known as metabarcoding. To obtain accurate estimates of diversity …
multiple taxa known as metabarcoding. To obtain accurate estimates of diversity …
An In silico approach for the evaluation of DNA barcodes
Background DNA barcoding is a key tool for assessing biodiversity in both taxonomic and
environmental studies. Essential features of barcodes include their applicability to a wide …
environmental studies. Essential features of barcodes include their applicability to a wide …
Underestimation of species richness in Neotropical frogs revealed by mtDNA analyses
Background Amphibians are rapidly vanishing. At the same time, it is most likely that the
number of amphibian species is highly underestimated. Recent DNA barcoding work has …
number of amphibian species is highly underestimated. Recent DNA barcoding work has …
The use of mean instead of smallest interspecific distances exaggerates the size of the “barcoding gap” and leads to misidentification
DNA barcoding is one of the best funded and most visible large-scale initiatives in
systematic biology and has generated both much interest and controversy. DNA barcoding …
systematic biology and has generated both much interest and controversy. DNA barcoding …
Deciphering amphibian diversity through DNA barcoding: chances and challenges
Amphibians globally are in decline, yet there is still a tremendous amount of unrecognized
diversity, calling for an acceleration of taxonomic exploration. This process will be greatly …
diversity, calling for an acceleration of taxonomic exploration. This process will be greatly …
Analysis of Australian fur seal diet by pyrosequencing prey DNA in faeces
DNA‐based techniques have proven useful for defining trophic links in a variety of
ecosystems and recently developed sequencing technologies provide new opportunities for …
ecosystems and recently developed sequencing technologies provide new opportunities for …
Relationship between morphological taxonomy and molecular divergence within Crustacea: proposal of a molecular threshold to help species delimitation
With today's technology for production of molecular sequences, DNA taxonomy and
barcoding arose as a new tool for evolutionary biology and ecology. However, their validities …
barcoding arose as a new tool for evolutionary biology and ecology. However, their validities …