Metabolic and signaling biochemical pathways have numerous steps that involve the
hydrolytic cleavage of peptide or phosphate ester bonds. Although both types of bonds are …

Numerous studies, both in enzymatic and nonenzymatic catalysis, have been undertaken to
understand the way by which metal ions, especially zinc ions, promote the hydrolysis of …

Interactions between molecules are ubiquitous and occur in our bodies, the food we eat, the
air we breathe, and myriad additional contexts. Although numerous tools are available for …

Pairs of Au nanoparticles have recently been proposed as “plasmon rulers” based on the
dependence of their light scattering on the interparticle distance. Preliminary work has …

Restriction endonucleases are enzymes which recognize short DNA sequences and cleave
the DNA in both strands. Depending on the enzymological properties different types are …

PI-SceI is a bifunctional yeast protein that propagates its mobile gene by catalyzing protein
splicing and site-specific DNA double-strand cleavage. Here, we report the 2.4 Å crystal …

The LAGLIDADG and HNH families of site-specific DNA endonucleases encoded by viruses,
bacteriophages as well as archaeal, eucaryotic nuclear and organellar genomes are …

The cytosol of the cell contains high concentrations of small and large macromolecules, but
experimental data are often obtained in dilute solutions that do not reflect in vivo conditions …

The 2.15-Å resolution cocrystal structure of Eco RV endonuclease mutant T93A complexed
with DNA and Ca2+ ions reveals two divalent metals bound in one of the active sites. One of …

TheSfiI endonuclease cleaves DNA by a mechanism that differs from other restriction
enzymes. While most restriction enzymes are dimeric proteins that interact with a single DNA …