Cytokines: from clinical significance to quantification

C Liu, D Chu, K Kalantar‐Zadeh, J George… - Advanced …, 2021 - Wiley Online Library
Cytokines are critical mediators that oversee and regulate immune and inflammatory
responses via complex networks and serve as biomarkers for many diseases. Quantification …

Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles

G Bordanaba-Florit, F Royo, SG Kruglik… - Nature …, 2021 - nature.com
Extracellular vesicles (EVs) are heterogeneous lipid containers with a complex molecular
cargo comprising several populations with unique roles in biological processes. These …

Roadmap on label‐free super‐resolution imaging

VN Astratov, YB Sahel, YC Eldar… - Laser & photonics …, 2023 - Wiley Online Library
Label‐free super‐resolution (LFSR) imaging relies on light‐scattering processes in
nanoscale objects without a need for fluorescent (FL) staining required in super‐resolved FL …

Characterizing extracellular vesicles and their diverse RNA contents

EM Veziroglu, GI Mias - Frontiers in genetics, 2020 - frontiersin.org
Cells release nanometer-scale, lipid bilayer-enclosed biomolecular packages (extracellular
vesicles; EVs) into their surrounding environment. EVs are hypothesized to be intercellular …

Tetraspanins are unevenly distributed across single extracellular vesicles and bias sensitivity to multiplexed cancer biomarkers

RR Mizenko, T Brostoff, T Rojalin, HJ Koster… - Journal of …, 2021 - Springer
Background Tetraspanin expression of extracellular vesicles (EVs) is often used as a
surrogate for their detection and classification, a practice that typically assumes their …

Scattering-based light microscopy: from metal nanoparticles to single proteins

L Priest, JS Peters, P Kukura - Chemical reviews, 2021 - ACS Publications
Our ability to detect, image, and quantify nanoscopic objects and molecules with visible light
has undergone dramatic improvements over the past few decades. While fluorescence has …

Bond-selective imaging by optically sensing the mid-infrared photothermal effect

Y Bai, J Yin, JX Cheng - Science advances, 2021 - science.org
Mid-infrared (IR) spectroscopic imaging using inherent vibrational contrast has been broadly
used as a powerful analytical tool for sample identification and characterization. However …

Elucidating methods for isolation and quantification of exosomes: a review

TK Kurian, S Banik, D Gopal, S Chakrabarti… - Molecular …, 2021 - Springer
Exosomes are the smallest extracellular vesicles present in most of the biological fluids.
They are found to play an important role in cell signaling, immune response, tumor …

Separation, characterization, and standardization of extracellular vesicles for drug delivery applications

D Buschmann, V Mussack, JB Byrd - Advanced drug delivery reviews, 2021 - Elsevier
Extracellular vesicles (EVs) are membranous nanovesicles secreted from living cells,
shuttling macromolecules in intercellular communication and potentially possessing intrinsic …

Understanding exosomes: Part 1—Characterization, quantification and isolation techniques

RJ Miron, Y Zhang - Periodontology 2000, 2024 - Wiley Online Library
Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells
with a diameter in the range of 30–150 nm. Their use has gained great momentum recently …