Top-down proteomics

DS Roberts, JA Loo, YO Tsybin, X Liu, S Wu… - Nature Reviews …, 2024‏ - nature.com
Proteoforms, which arise from post-translational modifications, genetic polymorphisms and
RNA splice variants, play a pivotal role as drivers in biology. Understanding proteoforms is …

Top-down proteomics and the challenges of true proteoform characterization

A Po, CE Eyers - Journal of proteome research, 2023‏ - ACS Publications
Top-down proteomics (TDP) aims to identify and profile intact protein forms (proteoforms)
extracted from biological samples. True proteoform characterization requires that both the …

Defining proteoform-specific interactions for drug targeting in a native cell signalling environment

CA Lutomski, JL Bennett, TJ El-Baba, D Wu… - Nature Chemistry, 2025‏ - nature.com
Understanding the dynamics of membrane protein–ligand interactions within a native lipid
bilayer is a major goal for drug discovery. Typically, cell-based assays are used, however …

Mass spectrometry-intensive top-down proteomics: an update on technology advancements and biomedical applications

T Xu, Q Wang, Q Wang, L Sun - Analytical Methods, 2024‏ - pubs.rsc.org
Proteoforms are all forms of protein molecules from the same gene because of variations at
the DNA, RNA, and protein levels, eg, alternative splicing and post-translational …

Recent progress in quantitative phosphoproteomics

K Zittlau, P Nashier, C Cavarischia-Rega… - Expert Review of …, 2023‏ - Taylor & Francis
Introduction Protein phosphorylation is a critical post-translational modification involved in
the regulation of numerous cellular processes from signal transduction to modulation of …

Native Top-Down Mass Spectrometry for Characterizing Sarcomeric Proteins Directly from Cardiac Tissue Lysate

EA Chapman, BH Li, B Krichel, HJ Chan… - Journal of the …, 2024‏ - ACS Publications
Native top-down mass spectrometry (nTDMS) has emerged as a powerful structural biology
tool that can localize post-translational modifications (PTMs), explore ligand-binding …

The 2023 report on the Proteome from the HUPO Human Proteome Project

GS Omenn, L Lane, CM Overall… - Journal of proteome …, 2024‏ - ACS Publications
Since 2010, the Human Proteome Project (HPP), the flagship initiative of the Human
Proteome Organization (HUPO), has pursued two goals:(1) to credibly identify the protein …

Post‐translational modifications of proteins in cardiovascular diseases examined by proteomic approaches

M Stastna - The FEBS Journal, 2025‏ - Wiley Online Library
Over 400 different types of post‐translational modifications (PTMs) have been reported and
over 200 various types of PTMs have been discovered using mass spectrometry (MS) …

Post‐translational modifications of vertebrate striated muscle myosin heavy chains

PN Morales, AN Coons, AJ Koopman, S Patel… - …, 2024‏ - Wiley Online Library
Post‐translational modifications (PTMs) play a crucial role in regulating the function of many
sarcomeric proteins, including myosin. Myosins comprise a family of motor proteins that play …

Multi‐omic analyses and network biology in cardiovascular disease

CJ Reitz, U Kuzmanov, AO Gramolini - Proteomics, 2023‏ - Wiley Online Library
Heart disease remains a leading cause of death in North America and worldwide. Despite
advances in therapies, the chronic nature of cardiovascular diseases ultimately results in …