[HTML][HTML] Enzyme stability and activity in non-aqueous reaction systems: a mini review

S Wang, X Meng, H Zhou, Y Liu, F Secundo, Y Liu - Catalysts, 2016 - mdpi.com
Enormous interest in biocatalysis in non-aqueous phase has recently been triggered due to
the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water …

Overview of the stability of α-chymotrypsin in different solvent media

A Kumar, P Venkatesu - Chemical Reviews, 2012 - ACS Publications
1. INTRODUCTION The ability of amino acids to form a three-dimensional (3D) protein
structure is significantly interesting because it combines the aspects of biophysical …

Proteomics of integral membrane proteins theory and application

AE Speers, CC Wu - Chemical reviews, 2007 - ACS Publications
Integral membrane proteins (IMPs) lie at the critical junctions between intracellular
compartments and cells and their environment. As such, IMPs are in a unique position to …

Understanding Structure−Stability Relationships of Candida antartica Lipase B in Ionic Liquids

T De Diego, P Lozano, S Gmouh, M Vaultier… - …, 2005 - ACS Publications
Two different water-immiscible ionic liquids (ILs), 1-ethyl-3-methylimidizolium bis
(trifluoromethylsulfonyl) imide and butyltrimethylammonium bis (trifluoromethylsulfonyl) …

Fluorescence and CD spectroscopic analysis of the α‐chymotrypsin stabilization by the ionic liquid, 1‐ethyl‐3‐methylimidazolium bis [(trifluoromethyl) sulfonyl] amide

T De Diego, P Lozano, S Gmouh… - Biotechnology and …, 2004 - Wiley Online Library
The stability of α‐chymotrypsin in the ionic liquid, 1‐ethyl‐3‐methyl‐imidizolium bis
[(trifluoromethyl) sulfonyl] amide ([emim][NTf2]), was studied at 30 and 50° C and compared …

Efficient and specific trypsin digestion of microgram to nanogram quantities of proteins in organic− aqueous solvent systems

MB Strader, DL Tabb, WJ Hervey, C Pan… - Analytical …, 2006 - ACS Publications
Mass spectrometry-based identification of the components of multiprotein complexes often
involves solution-phase proteolytic digestion of the complex. The affinity purification of …

Homogeneous biocatalysis in organic solvents and water-organic mixtures

GR Castro, T Knubovets - Critical Reviews in Biotechnology, 2003 - Taylor & Francis
Biocatalysis in non-aqueous media has undergone tremendous development during the last
decade, and numerous reactions have been introduced and optimized for synthetic …

Influence of osmolytes and denaturants on the structure and enzyme activity of α-chymotrypsin

P Attri, P Venkatesu, MJ Lee - The Journal of Physical Chemistry …, 2010 - ACS Publications
Enzymes are very sensitive and highly complex systems, exhibiting a substantial degree of
structural variability in their folded state. In the presence of cosolvents, the fluctuations …

Structural characterization of α‑chymotrypsin after binding to curcumin: Spectroscopic and computational analysis of their binding mechanism

S Farhadian, B Shareghi, S Asgharzadeh… - Journal of Molecular …, 2019 - Elsevier
Food has a major role in sickness, traditional medicine, and curing. Curcuma longa L. is one
of these medicinal plants. Curcuma longa L., a member of the ginger family, grows in tropical …

Osmolyte counteracts urea-induced denaturation of α-chymotrypsin

P Venkatesu, MJ Lee, H Lin - The Journal of Physical Chemistry B, 2009 - ACS Publications
The stability of proteins is reduced by urea, which is methylamine and nonprotecting
osmolyte; eventually urea destabilizes the activity and function and alters the structure of …