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[HTML][HTML] Enzyme stability and activity in non-aqueous reaction systems: a mini review
S Wang, X Meng, H Zhou, Y Liu, F Secundo, Y Liu - Catalysts, 2016 - mdpi.com
Enormous interest in biocatalysis in non-aqueous phase has recently been triggered due to
the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water …
the merits of good enantioselectivity, reverse thermodynamic equilibrium, and no water …
Overview of the stability of α-chymotrypsin in different solvent media
1. INTRODUCTION The ability of amino acids to form a three-dimensional (3D) protein
structure is significantly interesting because it combines the aspects of biophysical …
structure is significantly interesting because it combines the aspects of biophysical …
Proteomics of integral membrane proteins theory and application
AE Speers, CC Wu - Chemical reviews, 2007 - ACS Publications
Integral membrane proteins (IMPs) lie at the critical junctions between intracellular
compartments and cells and their environment. As such, IMPs are in a unique position to …
compartments and cells and their environment. As such, IMPs are in a unique position to …
Understanding Structure−Stability Relationships of Candida antartica Lipase B in Ionic Liquids
Two different water-immiscible ionic liquids (ILs), 1-ethyl-3-methylimidizolium bis
(trifluoromethylsulfonyl) imide and butyltrimethylammonium bis (trifluoromethylsulfonyl) …
(trifluoromethylsulfonyl) imide and butyltrimethylammonium bis (trifluoromethylsulfonyl) …
Fluorescence and CD spectroscopic analysis of the α‐chymotrypsin stabilization by the ionic liquid, 1‐ethyl‐3‐methylimidazolium bis [(trifluoromethyl) sulfonyl] amide
The stability of α‐chymotrypsin in the ionic liquid, 1‐ethyl‐3‐methyl‐imidizolium bis
[(trifluoromethyl) sulfonyl] amide ([emim][NTf2]), was studied at 30 and 50° C and compared …
[(trifluoromethyl) sulfonyl] amide ([emim][NTf2]), was studied at 30 and 50° C and compared …
Efficient and specific trypsin digestion of microgram to nanogram quantities of proteins in organic− aqueous solvent systems
Mass spectrometry-based identification of the components of multiprotein complexes often
involves solution-phase proteolytic digestion of the complex. The affinity purification of …
involves solution-phase proteolytic digestion of the complex. The affinity purification of …
Homogeneous biocatalysis in organic solvents and water-organic mixtures
GR Castro, T Knubovets - Critical Reviews in Biotechnology, 2003 - Taylor & Francis
Biocatalysis in non-aqueous media has undergone tremendous development during the last
decade, and numerous reactions have been introduced and optimized for synthetic …
decade, and numerous reactions have been introduced and optimized for synthetic …
Influence of osmolytes and denaturants on the structure and enzyme activity of α-chymotrypsin
Enzymes are very sensitive and highly complex systems, exhibiting a substantial degree of
structural variability in their folded state. In the presence of cosolvents, the fluctuations …
structural variability in their folded state. In the presence of cosolvents, the fluctuations …
Structural characterization of α‑chymotrypsin after binding to curcumin: Spectroscopic and computational analysis of their binding mechanism
Food has a major role in sickness, traditional medicine, and curing. Curcuma longa L. is one
of these medicinal plants. Curcuma longa L., a member of the ginger family, grows in tropical …
of these medicinal plants. Curcuma longa L., a member of the ginger family, grows in tropical …
Osmolyte counteracts urea-induced denaturation of α-chymotrypsin
The stability of proteins is reduced by urea, which is methylamine and nonprotecting
osmolyte; eventually urea destabilizes the activity and function and alters the structure of …
osmolyte; eventually urea destabilizes the activity and function and alters the structure of …