Sources, resolution and physiological relevance of R-loops and RNA–DNA hybrids

E Petermann, L Lan, L Zou - Nature reviews Molecular cell biology, 2022 - nature.com
RNA–DNA hybrids are generated during transcription, DNA replication and DNA repair and
are crucial intermediates in these processes. When RNA–DNA hybrids are stably formed in …

Human PCNA structure, function, and interactions

A González-Magaña, FJ Blanco - Biomolecules, 2020 - mdpi.com
Proliferating cell nuclear antigen (PCNA) is an essential factor in DNA replication and repair.
It forms a homotrimeric ring that embraces the DNA and slides along it, anchoring DNA …

Direct visualization of transcription-replication conflicts reveals post-replicative DNA: RNA hybrids

H Stoy, K Zwicky, D Kuster, KS Lang… - Nature Structural & …, 2023 - nature.com
Transcription-replication collisions (TRCs) are crucial determinants of genome instability. R-
loops were linked to head-on TRCs and proposed to obstruct replication fork progression …

RNase H2, mutated in Aicardi‐Goutières syndrome, resolves co-transcriptional R-loops to prevent DNA breaks and inflammation

A Cristini, M Tellier, F Constantinescu, C Accalai… - Nature …, 2022 - nature.com
RNase H2 is a specialized enzyme that degrades RNA in RNA/DNA hybrids and deficiency
of this enzyme causes a severe neuroinflammatory disease, Aicardi Goutières syndrome …

Replication-coupled DNA repair

D Cortez - Molecular cell, 2019 - cell.com
The replisome quickly and accurately copies billions of DNA bases each cell division cycle.
However, it can make errors, especially when the template DNA is damaged. In these cases …

[HTML][HTML] Enzymatic removal of ribonucleotides from DNA is essential for mammalian genome integrity and development

MAM Reijns, B Rabe, RE Rigby, P Mill, KR Astell… - Cell, 2012 - cell.com
The presence of ribonucleotides in genomic DNA is undesirable given their increased
susceptibility to hydrolysis. Ribonuclease (RNase) H enzymes that recognize and process …

[HTML][HTML] Functions of replication protein A as a sensor of R loops and a regulator of RNaseH1

HD Nguyen, T Yadav, S Giri, B Saez, TA Graubert… - Molecular cell, 2017 - cell.com
R loop, a transcription intermediate containing RNA: DNA hybrids and displaced single-
stranded DNA (ssDNA), has emerged as a major source of genomic instability. RNaseH1 …

Regulation of PCNA–protein interactions for genome stability

N Mailand, I Gibbs-Seymour… - Nature reviews Molecular …, 2013 - nature.com
Proliferating cell nuclear antigen (PCNA) has a central role in promoting faithful DNA
replication, providing a molecular platform that facilitates the myriad protein–protein and …

Nascent chromatin capture proteomics determines chromatin dynamics during DNA replication and identifies unknown fork components

C Alabert, JC Bukowski-Wills, SB Lee… - Nature cell …, 2014 - nature.com
To maintain genome function and stability, DNA sequence and its organization into
chromatin must be duplicated during cell division. Understanding how entire chromosomes …

[HTML][HTML] Counteracting chromatin effects of a splicing-correcting antisense oligonucleotide improves its therapeutic efficacy in spinal muscular atrophy

LE Marasco, G Dujardin, R Sousa-Luís, YH Liu… - Cell, 2022 - cell.com
Spinal muscular atrophy (SMA) is a motor-neuron disease caused by mutations of the SMN1
gene. The human paralog SMN2, whose exon 7 (E7) is predominantly skipped, cannot …