Strong production of recombinant proteins interferes with cellular processes in many ways.
Drainage of precursors and energy urges the cell to readjust metabolic fluxes and enzyme …

The demand for therapeutic recombinant proteins is set to see a significant increase over the
next few years. As a consequence, the processes used to produce these proteins must be …

Shake‐flask cultures are widely used for screening of high producing strains. To select
suitable strains for production scale, cultivation parameters should be applied that provide …

Recombinant proteins have broad applications. However, there is a lack of a recombinant
protein expression system specifically for large-scale production in anaerobic hosts. Here …

Oxygen transfer (OT) effects on benzaldehyde lyase (BAL) production by recombinant
Escherichia coli carrying pUC18:: bal gene were investigated on a defined medium with …

A commercial process was developed for producing a recombinant vaccine against
hydatidosis in farm animals. The vaccine antigen consisting of a surface protein of the …

By a sulfite oxidation method, oxygen transfer rates (OTRs) were determined in 11 types of
culture vessels from 2.8‐L Fernbach (FB) flasks to 96‐, 48‐, and 24‐well square deepwell …

Abstract Mucopolysaccharidosis IVA (MPS IVA) is an autosomal recessive disorder caused
by N-acetylgalactosamine-6-sulfate sulfatase (GALNS) deficiency. Currently no effective …

Escherichia coli is the main bacterial producer of heterologous proteins. The current
production strategies aim at growing the bacteria to high density in order to achieve high …

Escherichia coli is the most applied organism for the production of recombinant gene
products. The review summarizes systems and strategies which are applied, and evaluates …