Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
Fluorescence lifetime imaging microscopy
Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique offering profound
insights into a broad spectrum of biological processes such as metabolic imaging, protein …
insights into a broad spectrum of biological processes such as metabolic imaging, protein …
A polarity‐sensitive ratiometric fluorescence probe for monitoring changes in lipid droplets and nucleus during ferroptosis
Ferroptosis regulates cell death through reactive oxygen species (ROS)‐associated lipid
peroxide accumulation, which is expected to affect the structure and polarity of lipid droplets …
peroxide accumulation, which is expected to affect the structure and polarity of lipid droplets …
Deep learning in medical image registration: a survey
The establishment of image correspondence through robust image registration is critical to
many clinical tasks such as image fusion, organ atlas creation, and tumor growth monitoring …
many clinical tasks such as image fusion, organ atlas creation, and tumor growth monitoring …
DeepSTORM3D: dense 3D localization microscopy and PSF design by deep learning
An outstanding challenge in single-molecule localization microscopy is the accurate and
precise localization of individual point emitters in three dimensions in densely labeled …
precise localization of individual point emitters in three dimensions in densely labeled …
High-fidelity carbon dots polarity probes: revealing the heterogeneity of lipids in oncology
J Hu, Y Sun, X Geng, J Wang, Y Guo, L Qu… - Light: Science & …, 2022 - nature.com
Polarity is an integral microenvironment parameter in biological systems closely associated
with a multitude of cellular processes. Abnormal polarity variations accompany the initiation …
with a multitude of cellular processes. Abnormal polarity variations accompany the initiation …
Label-free sensing of cells with fluorescence lifetime imaging: The quest for metabolic heterogeneity
EA Shirshin, MV Shirmanova… - Proceedings of the …, 2022 - National Acad Sciences
Molecular, morphological, and physiological heterogeneity is the inherent property of cells
which governs differences in their response to external influence. Tumor cell metabolic …
which governs differences in their response to external influence. Tumor cell metabolic …
Fast identification of fluorescent components in three-dimensional excitation-emission matrix fluorescence spectra via deep learning
Three-dimensional excitation-emission matrix (3D-EEM) fluorescence spectroscopy has
been widely applied to detect the fluorescent components in samples from natural water …
been widely applied to detect the fluorescent components in samples from natural water …
Polarity-sensitive and membrane-specific probe quantitatively monitoring ferroptosis through fluorescence lifetime imaging
S Wu, Y Yan, H Hou, Z Huang, D Li, X Zhang… - Analytical …, 2022 - ACS Publications
As a new form of regulated cell death, ferroptosis is closely related to various diseases. To
interpret this biological behavior and monitor related pathological processes, it is necessary …
interpret this biological behavior and monitor related pathological processes, it is necessary …
Deep learning for biospectroscopy and biospectral imaging: state-of-the-art and perspectives
With the advances in instrumentation and sampling techniques, there is an explosive growth
of data from molecular and cellular samples. The call to extract more information from the …
of data from molecular and cellular samples. The call to extract more information from the …