The CRISPR-Cas9 system has become the most promising and versatile tool for genetic
manipulation applications. Albeit the technology has been broadly adopted by both …

Continuing crop domestication/redomestication and modification is a key determinant of the
adaptation and fulfillment of the food requirements of an exploding global population under …

In genome editing with CRISPR–Cas9, transgene integration often remains challenging.
Here, we present an approach for increasing the efficiency of transgene integration by …

Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations
found in humans with genetic disorders. However, there is a lack of genetic engineering …

Methods for sensitive and high-throughput evaluation of CRISPR RNA-guided nucleases
(RGNs) off-targets (OTs) are essential for advancing RGN-based gene therapies. Here we …

Albumin accumulation in tumours could reflect a role of albumin in transport of endogenous
nutrient cargos required for cellular growth and not just a suggested source of amino acids; …

A variety of inherited or multifactorial ocular diseases call for novel treatment paradigms. The
newly developed genome editing technology, CRISPR, has shown great promise in treating …

Breakthroughs in the development of programmable site-specific nucleases, including zinc-
finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) …

Control of biological populations is an ongoing challenge in many fields, including
agriculture, biodiversity, ecological preservation, pest control, and the spread of disease. In …

Cas9 is an endonuclease that can be programed to autonomously deliver diverse effectors
to specified genetic addresses. High-resolution structures of this protein and its associated …